1kd4: Difference between revisions
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New page: left|200px<br /><applet load="1kd4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kd4, resolution 1.85Å" /> '''The Crystal Structur... |
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== | ==The Crystal Structure of r(GGUCACAGCCC)2, Barium form== | ||
Crystals of small RNAs, which regularly diffract to very high resolution, can often be readily obtained. Unfortunately, for some RNAs the | <StructureSection load='1kd4' size='340' side='right'caption='[[1kd4]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1kd4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KD4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KD4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kd4 OCA], [https://pdbe.org/1kd4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kd4 RCSB], [https://www.ebi.ac.uk/pdbsum/1kd4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kd4 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Crystals of small RNAs, which regularly diffract to very high resolution, can often be readily obtained. Unfortunately, for some RNAs the conformations adopted in the crystalline form are different from those found in solution. For example, short RNAs that form hairpins in solution virtually never crystallize thus; rather, they form duplexes. Nevertheless, these unintended structures have contributed greatly to the understanding of RNA structure. In a similar occurrence, the homodimer r(GGUCACAGCCC)(2) has been crystallized from an 11-mer/12-mer heteroduplex, r(GGCUGAAGUCCG)/r(GGUCACAGCCC). This surprising phenomenon was observed under a variety of crystallization conditions. The structure of the homoduplex was determined from crystals that differed in the precipitant used and the type of metal present. In all cases, the resulting homoduplexes contain ten base pairings, of which the central six are non-canonical pairings. In two of the variants, ordered metal-binding sites were observed: two equivalent octacoordinate Tl(+) sites in one and two equivalent nanocoordinate Ba(2+) sites in another. | |||
Crystal structures of r(GGUCACAGCCC)2.,Kacer V, Scaringe SA, Scarsdale JN, Rife JP Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):423-32. Epub 2003, Feb 21. PMID:12595698<ref>PMID:12595698</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 1kd4" style="background-color:#fffaf0;"></div> | ||
[[Category: Kacer | == References == | ||
[[Category: Rife | <references/> | ||
[[Category: Scaringe | __TOC__ | ||
[[Category: Scarsdale | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Kacer V]] | |||
[[Category: Rife JP]] | |||
[[Category: Scaringe SA]] | |||
[[Category: Scarsdale JN]] | |||
Latest revision as of 08:05, 4 March 2026
The Crystal Structure of r(GGUCACAGCCC)2, Barium form
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