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New page: left|200px<br /><applet load="1x31" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x31, resolution 2.15Å" /> '''Crystal Structure of...
 
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[[Image:1x31.gif|left|200px]]<br /><applet load="1x31" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1x31, resolution 2.15&Aring;" />
'''Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96'''<br />


==Overview==
==Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96==
Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric, enzyme. Here we report the crystal structures of the enzyme in complex, with dimethylglycine and folinic acid. The alpha subunit is composed of, two domains, contains NAD(+), and binds folinic acid. The beta subunit, contains dimethylglycine, FAD, and FMN, and these flavins are, approximately 10A apart. The gamma subunit is in contact with two domains, of alpha subunit and has possibly a folate-binding structure. The delta, subunit contains a single atom of zinc and has a Cys(3)His zinc finger, structure. Based on the structures determined and on the previous works, the structure-function relationship on the heterotetrameric sarcosine, oxidase is discussed.
<StructureSection load='1x31' size='340' side='right'caption='[[1x31]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1x31]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_sp._U-96 Corynebacterium sp. U-96]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X31 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMG:N,N-DIMETHYLGLYCINE'>DMG</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x31 OCA], [https://pdbe.org/1x31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x31 RCSB], [https://www.ebi.ac.uk/pdbsum/1x31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x31 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q50LF0_9CORY Q50LF0_9CORY]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x3/1x31_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x31 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1X31 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Corynebacterium_sp. Corynebacterium sp.] with ZN, SO4, FAD, NAD, FMN and DMG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Sarcosine_oxidase Sarcosine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.1 1.5.3.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1X31 OCA].
*[[Sarcosine oxidase|Sarcosine oxidase]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96., Ida K, Moriguchi T, Suzuki H, Biochem Biophys Res Commun. 2005 Jul 29;333(2):359-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15946648 15946648]
[[Category: Corynebacterium sp. U-96]]
[[Category: Corynebacterium sp.]]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Ida K]]
[[Category: Sarcosine oxidase]]
[[Category: Moriguchi T]]
[[Category: Ida, K.]]
[[Category: Suzuki H]]
[[Category: Moriguchi, T.]]
[[Category: Suzuki, H.]]
[[Category: DMG]]
[[Category: FAD]]
[[Category: FMN]]
[[Category: NAD]]
[[Category: SO4]]
[[Category: ZN]]
[[Category: flavoenzyme]]
[[Category: heterotetrameric sarcosine oxidase]]
 
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Retrieved from "https://proteopedia.org/1x31"