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[[Image:2cft.gif|left|200px]]<br />
<applet load="2cft" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2cft, resolution 1.80&Aring;" />
'''CRYSTAL STRUCTURE OF HUMAN PYRIDOXAL 5'-PHOSPHATE PHOSPHATASE WITH ITS SUBSTRATE'''<br />


==About this Structure==
==Crystal structure of human pyridoxal 5'-phosphate phosphatase with its substrate==
2CFT is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with CA and PLP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.74 3.1.3.74]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFT OCA]].  
<StructureSection load='2cft' size='340' side='right'caption='[[2cft]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2cft]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CFT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cft OCA], [https://pdbe.org/2cft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cft RCSB], [https://www.ebi.ac.uk/pdbsum/2cft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cft ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PLPP_HUMAN PLPP_HUMAN] Protein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho-tyrosine. Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PMP) and pyridoxine 5'-phosphate (PNP), with a highest activity with PLP followed by PNP.<ref>PMID:14522954</ref> <ref>PMID:15580268</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/2cft_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cft ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cho, H.J.]]
[[Category: Cho HJ]]
[[Category: Kang, B.S.]]
[[Category: Kang BS]]
[[Category: Kim, K.J.]]
[[Category: Kim KJ]]
[[Category: Kwon, O.S.]]
[[Category: Kwon OS]]
[[Category: CA]]
[[Category: PLP]]
[[Category: chronophin]]
[[Category: had family]]
[[Category: hydrolase]]
[[Category: magnesium]]
[[Category: metal-binding]]
[[Category: phosphatase]]
[[Category: pyridoxal phosphate]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:35:57 2007''

Latest revision as of 08:25, 9 April 2025

Crystal structure of human pyridoxal 5'-phosphate phosphatase with its substrate

2cft, resolution 1.80Å

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