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New page: left|200px<br /><applet load="1t1o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t1o" /> '''Components of the control 70S ribosome to pr...
 
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[[Image:1t1o.gif|left|200px]]<br /><applet load="1t1o" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1t1o" />
'''Components of the control 70S ribosome to provide reference for the RRF binding site'''<br />


==Overview==
==Components of the control 70S ribosome to provide reference for the RRF binding site==
After the termination step of protein synthesis, a deacylated tRNA and, mRNA remain associated with the ribosome. The ribosome-recycling factor, (RRF), together with elongation factor G (EF-G), disassembles this, posttermination complex into mRNA, tRNA, and the ribosome. We have, obtained a three-dimensional cryo-electron microscopic map of a complex of, the Escherichia coli 70S ribosome and RRF. We find that RRF interacts, mainly with the segments of the large ribosomal subunit's (50S) rRNA, helices that are involved in the formation of two central intersubunit, bridges, B2a and B3. The binding of RRF induces considerable, conformational changes in some of the functional domains of the ribosome., As compared to its binding position derived previously by hydroxyl radical, probing study, we find that RRF binds further inside the intersubunit, space of the ribosome such that the tip of its domain I is shifted (by, approximately 13 A) toward protein L5 within the central protuberance of, the 50S subunit, and domain II is oriented more toward the small ribosomal, subunit (30S). Overlapping binding sites of RRF, EF-G, and the P-site tRNA, suggest that the binding of EF-G would trigger the removal of deacylated, tRNA from the P site by moving RRF toward the ribosomal E site, and, subsequent removal of mRNA may be induced by a shift in the position of, 16S rRNA helix 44, which harbors part of the mRNA.
<SX load='1t1o' size='340' side='right' viewer='molstar' caption='[[1t1o]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1t1o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T1O FirstGlance]. <br>
1T1O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T1O OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1o OCA], [https://pdbe.org/1t1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t1o RCSB], [https://www.ebi.ac.uk/pdbsum/1t1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1o ProSAT]</span></td></tr>
==Reference==
</table>
Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications., Agrawal RK, Sharma MR, Kiel MC, Hirokawa G, Booth TM, Spahn CM, Grassucci RA, Kaji A, Frank J, Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8900-5. Epub 2004 Jun 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15178758 15178758]
__TOC__
</SX>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Agrawal, R.K.]]
[[Category: Agrawal RK]]
[[Category: Booth, T.M.]]
[[Category: Booth TM]]
[[Category: Frank, J.]]
[[Category: Frank J]]
[[Category: Grassucci, R.A.]]
[[Category: Grassucci RA]]
[[Category: Hirokawa, G.]]
[[Category: Hirokawa G]]
[[Category: Kaji, A.]]
[[Category: Kaji A]]
[[Category: Kiel, M.C.]]
[[Category: Kiel MC]]
[[Category: Sharma, M.R.]]
[[Category: Sharma MR]]
[[Category: Spahn, C.M.]]
[[Category: Spahn CM]]
[[Category: rrf binding position on the ribosome]]
 
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Latest revision as of 08:35, 14 February 2024

Components of the control 70S ribosome to provide reference for the RRF binding site

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