1kp7: Difference between revisions

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[[Image:1kp7.gif|left|200px]]<br /><applet load="1kp7" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1kp7.gif|left|200px]]<br /><applet load="1kp7" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1kp7" />

'''Conserved RNA Structure within the HCV IRES eIF3 Binding Site'''<br />

==Overview==

The hepatitis C virus (HCV) internal ribosome entry site (IRES) is, recognized specifically by the small ribosomal subunit and eukaryotic, initiation factor 3 (eIF3) before viral translation initiation. Using, extensive mutagenesis and structure probing analysis, we show that the, eIF3-binding domain of the HCV IRES contains an internal loop structure, (loop IIIb) and an adjacent mismatched helix that are important for, IRES-dependent initiation of translation. NMR studies reveal a unique, three-dimensional structure for this internal loop that is conserved, between viral isolates of varying primary sequence in this region. These, data indicate that internal loop IIIb may be an attractive target for, structure-based design of new antiviral agents.

The hepatitis C virus (HCV) internal ribosome entry site (IRES) is recognized specifically by the small ribosomal subunit and eukaryotic initiation factor 3 (eIF3) before viral translation initiation. Using extensive mutagenesis and structure probing analysis, we show that the eIF3-binding domain of the HCV IRES contains an internal loop structure (loop IIIb) and an adjacent mismatched helix that are important for IRES-dependent initiation of translation. NMR studies reveal a unique three-dimensional structure for this internal loop that is conserved between viral isolates of varying primary sequence in this region. These data indicate that internal loop IIIb may be an attractive target for structure-based design of new antiviral agents.

==About this Structure==

1KP7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1KP7 OCA].

1KP7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KP7 OCA].

==Reference==

Line 13:

[[Category: Protein complex]]

[[Category: Aboul-ela, F.]]

[[Category: Cole, P.T.]]

[[Category: Cole, P T.]]

[[Category: Collier, A.J.]]

[[Category: Collier, A J.]]

[[Category: Gallego, J.]]

[[Category: Harris, S.J.]]

[[Category: Harris, S J.]]

[[Category: Harrison, G.P.]]

[[Category: Harrison, G P.]]

[[Category: Klinck, R.]]

[[Category: Varani, G.]]

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[[Category: s-turn]]

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@@ Line 1: / Line 1: @@
-[[Image:1kp7.gif|left|200px]]<br /><applet load="1kp7" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1kp7.gif|left|200px]]<br /><applet load="1kp7" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1kp7" />
 '''Conserved RNA Structure within the HCV IRES eIF3 Binding Site'''<br />
 ==Overview==
-The hepatitis C virus (HCV) internal ribosome entry site (IRES) is, recognized specifically by the small ribosomal subunit and eukaryotic, initiation factor 3 (eIF3) before viral translation initiation. Using, extensive mutagenesis and structure probing analysis, we show that the, eIF3-binding domain of the HCV IRES contains an internal loop structure, (loop IIIb) and an adjacent mismatched helix that are important for, IRES-dependent initiation of translation. NMR studies reveal a unique, three-dimensional structure for this internal loop that is conserved, between viral isolates of varying primary sequence in this region. These, data indicate that internal loop IIIb may be an attractive target for, structure-based design of new antiviral agents.
+The hepatitis C virus (HCV) internal ribosome entry site (IRES) is recognized specifically by the small ribosomal subunit and eukaryotic initiation factor 3 (eIF3) before viral translation initiation. Using extensive mutagenesis and structure probing analysis, we show that the eIF3-binding domain of the HCV IRES contains an internal loop structure (loop IIIb) and an adjacent mismatched helix that are important for IRES-dependent initiation of translation. NMR studies reveal a unique three-dimensional structure for this internal loop that is conserved between viral isolates of varying primary sequence in this region. These data indicate that internal loop IIIb may be an attractive target for structure-based design of new antiviral agents.
 ==About this Structure==
-KP7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KP7 OCA].
+KP7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KP7 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Aboul-ela, F.]]
-[[Category: Cole, P.T.]]
+[[Category: Cole, P T.]]
-[[Category: Collier, A.J.]]
+[[Category: Collier, A J.]]
 [[Category: Gallego, J.]]
-[[Category: Harris, S.J.]]
+[[Category: Harris, S J.]]
-[[Category: Harrison, G.P.]]
+[[Category: Harrison, G P.]]
 [[Category: Klinck, R.]]
 [[Category: Varani, G.]]
@@ Line 28: / Line 28: @@
 [[Category: s-turn]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:42:40 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:29 2008''