1p59: Difference between revisions

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[[Image:1p59.gif|left|200px]]<br /><applet load="1p59" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1p59.gif|left|200px]]<br /><applet load="1p59" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1p59, resolution 2.50Å" />

'''Structure of a non-covalent Endonuclease III-DNA Complex'''<br />

==Overview==

Nearly all cells express proteins that confer resistance to the mutagenic, effects of oxidative DNA damage. The primary defense against the toxicity, of oxidative nucleobase lesions in DNA is the base-excision repair (BER), pathway. Endonuclease III (EndoIII) is a [4Fe-4S] cluster-containing DNA, glycosylase with repair activity specific for oxidized pyrimidine lesions, in duplex DNA. We have determined the crystal structure of a trapped, intermediate that represents EndoIII frozen in the act of repairing DNA., The structure of the protein-DNA complex provides insight into the ability, of EndoIII to recognize and repair a diverse array of oxidatively damaged, bases. This structure also suggests a rationale for the frequent, occurrence in certain human cancers of a specific mutation in the related, DNA repair protein MYH.

Nearly all cells express proteins that confer resistance to the mutagenic effects of oxidative DNA damage. The primary defense against the toxicity of oxidative nucleobase lesions in DNA is the base-excision repair (BER) pathway. Endonuclease III (EndoIII) is a [4Fe-4S] cluster-containing DNA glycosylase with repair activity specific for oxidized pyrimidine lesions in duplex DNA. We have determined the crystal structure of a trapped intermediate that represents EndoIII frozen in the act of repairing DNA. The structure of the protein-DNA complex provides insight into the ability of EndoIII to recognize and repair a diverse array of oxidatively damaged bases. This structure also suggests a rationale for the frequent occurrence in certain human cancers of a specific mutation in the related DNA repair protein MYH.

==About this Structure==

1P59 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with NA and SF4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1P59 OCA].

1P59 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=SF4:'>SF4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P59 OCA].

==Reference==

Line 13:

[[Category: Geobacillus stearothermophilus]]

[[Category: Protein complex]]

[[Category: Fromme, J.C.]]

[[Category: Fromme, J C.]]

[[Category: Verdine, G.L.]]

[[Category: Verdine, G L.]]

[[Category: NA]]

[[Category: SF4]]

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[[Category: iron-sulfur cluster]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov~~ 25 ~~02:01~~:~~01 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:14 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1p59.gif|left|200px]]<br /><applet load="1p59" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1p59.gif|left|200px]]<br /><applet load="1p59" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1p59, resolution 2.50&Aring;" />
 '''Structure of a non-covalent Endonuclease III-DNA Complex'''<br />
 ==Overview==
-Nearly all cells express proteins that confer resistance to the mutagenic, effects of oxidative DNA damage. The primary defense against the toxicity, of oxidative nucleobase lesions in DNA is the base-excision repair (BER), pathway. Endonuclease III (EndoIII) is a [4Fe-4S] cluster-containing DNA, glycosylase with repair activity specific for oxidized pyrimidine lesions, in duplex DNA. We have determined the crystal structure of a trapped, intermediate that represents EndoIII frozen in the act of repairing DNA., The structure of the protein-DNA complex provides insight into the ability, of EndoIII to recognize and repair a diverse array of oxidatively damaged, bases. This structure also suggests a rationale for the frequent, occurrence in certain human cancers of a specific mutation in the related, DNA repair protein MYH.
+Nearly all cells express proteins that confer resistance to the mutagenic effects of oxidative DNA damage. The primary defense against the toxicity of oxidative nucleobase lesions in DNA is the base-excision repair (BER) pathway. Endonuclease III (EndoIII) is a [4Fe-4S] cluster-containing DNA glycosylase with repair activity specific for oxidized pyrimidine lesions in duplex DNA. We have determined the crystal structure of a trapped intermediate that represents EndoIII frozen in the act of repairing DNA. The structure of the protein-DNA complex provides insight into the ability of EndoIII to recognize and repair a diverse array of oxidatively damaged bases. This structure also suggests a rationale for the frequent occurrence in certain human cancers of a specific mutation in the related DNA repair protein MYH.
 ==About this Structure==
-P59 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with NA and SF4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P59 OCA].
+P59 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=SF4:'>SF4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P59 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Geobacillus stearothermophilus]]
 [[Category: Protein complex]]
-[[Category: Fromme, J.C.]]
+[[Category: Fromme, J C.]]
-[[Category: Verdine, G.L.]]
+[[Category: Verdine, G L.]]
 [[Category: NA]]
 [[Category: SF4]]
@@ Line 22: / Line 22: @@
 [[Category: iron-sulfur cluster]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:01:01 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:14 2008''