1ti7: Difference between revisions
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New page: left|200px<br /><applet load="1ti7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ti7, resolution 1.70Å" /> '''CRYSTAL STRUCTURE OF... |
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[[Image:1ti7.jpg|left|200px]]<br /><applet load="1ti7" size=" | [[Image:1ti7.jpg|left|200px]]<br /><applet load="1ti7" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1ti7, resolution 1.70Å" /> | caption="1ti7, resolution 1.70Å" /> | ||
'''CRYSTAL STRUCTURE OF NMRA, A NEGATIVE TRANSCRIPTIONAL REGULATOR, IN COMPLEX WITH NADP AT 1.7A RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF NMRA, A NEGATIVE TRANSCRIPTIONAL REGULATOR, IN COMPLEX WITH NADP AT 1.7A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
NmrA, a transcription repressor involved in the regulation of nitrogen | NmrA, a transcription repressor involved in the regulation of nitrogen metabolism in Aspergillus nidulans,is a member of the short-chain dehydrogenase reductase superfamily. Isothermal titration calorimetry and differential scanning calorimetry have been used to show NmrA binds NAD+ and NADP+ with similar affinity (average KD 65 microM) but has a greatly reduced affinity for NADH and NADPH (average KD 6.0 mM). The structure of NmrA in a complex with NADP+ reveals how repositioning a His-37 side chain allows the different conformations of NAD+ and NADP+ to be accommodated. Modeling NAD(P)H into NmrA indicated that steric clashes, attenuation of electrostatic interactions, and loss of aromatic ring stacking can explain the differing affinities of NAD(P)+/NAD(P)H. The ability of NmrA to discriminate between the oxidized and reduced forms of the dinucleotides may be linked to a possible role in redox sensing. Isothermal titration calorimetry demonstrated that NmrA and a C-terminal fragment of the GATA transcription factor AreA interacted with a 1:1 stoichiometry and an apparent KD of 0.26 microM. NmrA was unable to bind the nitrogen metabolite repression signaling molecules ammonium or glutamine. | ||
==About this Structure== | ==About this Structure== | ||
1TI7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans] with K, CL, NAP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | 1TI7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans] with <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NAP:'>NAP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1PDS. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TI7 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cooper, A.]] | [[Category: Cooper, A.]] | ||
[[Category: Dodds, A | [[Category: Dodds, A L.]] | ||
[[Category: Hawkins, A | [[Category: Hawkins, A R.]] | ||
[[Category: Johnson, C.]] | [[Category: Johnson, C.]] | ||
[[Category: Lamb, H | [[Category: Lamb, H K.]] | ||
[[Category: Leslie, K.]] | [[Category: Leslie, K.]] | ||
[[Category: Nutley, M.]] | [[Category: Nutley, M.]] | ||
[[Category: Stammers, D | [[Category: Stammers, D K.]] | ||
[[Category: Thompson, P.]] | [[Category: Thompson, P.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: transcriptional regulation]] | [[Category: transcriptional regulation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:50 2008'' | ||
