MDM2: Difference between revisions
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==Experimental Research== | ==Experimental Research== | ||
Early work in the field of MDM2 overproduction in tumor cells was done by Raphael E. Pollok et. al. | Early work in the field of MDM2 overproduction in tumor cells was done by Raphael E. Pollok et. al. This research consisted of tests done with cancerous tumor tissue and analogous tissue. DNA amplification, mRNA creation, and protein creation were all areas of interest in determining what mechanism was being utilized for the overexertion of MDM2. The amplification of the DNA would show a DNA based increase in coding for the protein. An excess of mRNA would show the increase in protein was due to increased transcription factors for MDM2. The last possibility would be that the overproduction of MDM2 was an error in translation and only protein production was to blame. | ||
These factors were tested using Southern, Northern, and Western plots to analyze DNA, mRNA, and protein production respectively. These tests use targeting antigens to test for the presence of desired proteins. The results of these tests reveled the genetic amplification of the DNA was to blame for the increased of MDM2. Also evidence that p53 protein mutations affect MDM2 were found. These results are of great use to focus treatment possibilities and further research.[1] | |||
==Reference== | ==Reference== | ||
