1l2d: Difference between revisions

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[[Image:1l2d.gif|left|200px]]<br /><applet load="1l2d" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1l2d.gif|left|200px]]<br /><applet load="1l2d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1l2d, resolution 2.0Å" />

'''MutM (Fpg)-DNA Estranged Guanine Mismatch Recognition Complex'''<br />

==Overview==

MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating, base-excision repair of oxidized guanine residues in DNA. Here we report, five different crystal structures of MutM-DNA complexes that represent, different steps of the repair reaction cascade catalyzed by the protein, and also differ in the identity of the base opposite the lesion (the, 'estranged' base). These structures reveal that the MutM active site, performs the multiple steps of base-excision and 3' and 5' nicking with, minimal rearrangement of the DNA backbone.

MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating base-excision repair of oxidized guanine residues in DNA. Here we report five different crystal structures of MutM-DNA complexes that represent different steps of the repair reaction cascade catalyzed by the protein and also differ in the identity of the base opposite the lesion (the 'estranged' base). These structures reveal that the MutM active site performs the multiple steps of base-excision and 3' and 5' nicking with minimal rearrangement of the DNA backbone.

==About this Structure==

1L2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1L2D OCA].

1L2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2D OCA].

==Reference==

Line 13:

[[Category: Geobacillus stearothermophilus]]

[[Category: Protein complex]]

[[Category: Fromme, J.C.]]

[[Category: Fromme, J C.]]

[[Category: Verdine, G.L.]]

[[Category: Verdine, G L.]]

[[Category: ZN]]

[[Category: dna glycosylase]]

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[[Category: zinc finger]]

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@@ Line 1: / Line 1: @@
-[[Image:1l2d.gif|left|200px]]<br /><applet load="1l2d" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1l2d.gif|left|200px]]<br /><applet load="1l2d" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1l2d, resolution 2.0&Aring;" />
 '''MutM (Fpg)-DNA Estranged Guanine Mismatch Recognition Complex'''<br />
 ==Overview==
-MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating, base-excision repair of oxidized guanine residues in DNA. Here we report, five different crystal structures of MutM-DNA complexes that represent, different steps of the repair reaction cascade catalyzed by the protein, and also differ in the identity of the base opposite the lesion (the, 'estranged' base). These structures reveal that the MutM active site, performs the multiple steps of base-excision and 3' and 5' nicking with, minimal rearrangement of the DNA backbone.
+MutM is a bacterial 8-oxoguanine glycosylase responsible for initiating base-excision repair of oxidized guanine residues in DNA. Here we report five different crystal structures of MutM-DNA complexes that represent different steps of the repair reaction cascade catalyzed by the protein and also differ in the identity of the base opposite the lesion (the 'estranged' base). These structures reveal that the MutM active site performs the multiple steps of base-excision and 3' and 5' nicking with minimal rearrangement of the DNA backbone.
 ==About this Structure==
-L2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L2D OCA].
+L2D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2D OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Geobacillus stearothermophilus]]
 [[Category: Protein complex]]
-[[Category: Fromme, J.C.]]
+[[Category: Fromme, J C.]]
-[[Category: Verdine, G.L.]]
+[[Category: Verdine, G L.]]
 [[Category: ZN]]
 [[Category: dna glycosylase]]
@@ Line 20: / Line 20: @@
 [[Category: zinc finger]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:23:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:30 2008''