1tlr: Difference between revisions

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[[Image:1tlr.jpg|left|200px]]<br /><applet load="1tlr" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1tlr.jpg|left|200px]]<br /><applet load="1tlr" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1tlr" />

'''SOLUTION STRUCTURE OF TETRALOOP RECEPTOR RNA, NMR, 20 STRUCTURES'''<br />

==Overview==

The GAAA tetraloop receptor is an 11-nucleotide RNA sequence that, participates in the tertiary folding of a variety of large catalytic RNAs, by providing a specific binding site for GAAA tetraloops. Here we report, the solution structure of the isolated tetraloop receptor as solved by, multidimensional, heteronuclear magnetic resonance spectroscopy. The, internal loop of the tetraloop receptor has three adenosines stacked in a, cross-strand or zipper-like fashion. This arrangement produces a high, degree of base stacking within the asymmetric internal loop without, extrahelical bases or kinking the helix. Additional interactions within, the internal loop include a U. U mismatch pair and a G.U wobble pair. A, comparison with the crystal structure of the receptor RNA bound to its, tetraloop shows that a conformational change has to occur upon tetraloop, binding, which is in good agreement with previous biochemical data. A, model for an alternative binding site within the receptor is proposed, based on the NMR structure, phylogenetic data and previous, crystallographic structures of tetraloop interactions.

The GAAA tetraloop receptor is an 11-nucleotide RNA sequence that participates in the tertiary folding of a variety of large catalytic RNAs by providing a specific binding site for GAAA tetraloops. Here we report the solution structure of the isolated tetraloop receptor as solved by multidimensional, heteronuclear magnetic resonance spectroscopy. The internal loop of the tetraloop receptor has three adenosines stacked in a cross-strand or zipper-like fashion. This arrangement produces a high degree of base stacking within the asymmetric internal loop without extrahelical bases or kinking the helix. Additional interactions within the internal loop include a U. U mismatch pair and a G.U wobble pair. A comparison with the crystal structure of the receptor RNA bound to its tetraloop shows that a conformational change has to occur upon tetraloop binding, which is in good agreement with previous biochemical data. A model for an alternative binding site within the receptor is proposed based on the NMR structure, phylogenetic data and previous crystallographic structures of tetraloop interactions.

==About this Structure==

1TLR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1TLR OCA].

1TLR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLR OCA].

==Reference==

Solution structure of a GAAA tetraloop receptor RNA., Butcher SE, Dieckmann T, Feigon J, EMBO J. 1997 Dec 15;16(24):7490-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9405377 9405377]

[[Category: Protein complex]]

[[Category: Butcher, S.E.]]

[[Category: Butcher, S E.]]

[[Category: Dieckmann, T.]]

[[Category: Feigon, J.]]

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[[Category: tetraloop receptor]]

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@@ Line 1: / Line 1: @@
-[[Image:1tlr.jpg|left|200px]]<br /><applet load="1tlr" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1tlr.jpg|left|200px]]<br /><applet load="1tlr" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1tlr" />
 '''SOLUTION STRUCTURE OF TETRALOOP RECEPTOR RNA, NMR, 20 STRUCTURES'''<br />
 ==Overview==
-The GAAA tetraloop receptor is an 11-nucleotide RNA sequence that, participates in the tertiary folding of a variety of large catalytic RNAs, by providing a specific binding site for GAAA tetraloops. Here we report, the solution structure of the isolated tetraloop receptor as solved by, multidimensional, heteronuclear magnetic resonance spectroscopy. The, internal loop of the tetraloop receptor has three adenosines stacked in a, cross-strand or zipper-like fashion. This arrangement produces a high, degree of base stacking within the asymmetric internal loop without, extrahelical bases or kinking the helix. Additional interactions within, the internal loop include a U. U mismatch pair and a G.U wobble pair. A, comparison with the crystal structure of the receptor RNA bound to its, tetraloop shows that a conformational change has to occur upon tetraloop, binding, which is in good agreement with previous biochemical data. A, model for an alternative binding site within the receptor is proposed, based on the NMR structure, phylogenetic data and previous, crystallographic structures of tetraloop interactions.
+The GAAA tetraloop receptor is an 11-nucleotide RNA sequence that participates in the tertiary folding of a variety of large catalytic RNAs by providing a specific binding site for GAAA tetraloops. Here we report the solution structure of the isolated tetraloop receptor as solved by multidimensional, heteronuclear magnetic resonance spectroscopy. The internal loop of the tetraloop receptor has three adenosines stacked in a cross-strand or zipper-like fashion. This arrangement produces a high degree of base stacking within the asymmetric internal loop without extrahelical bases or kinking the helix. Additional interactions within the internal loop include a U. U mismatch pair and a G.U wobble pair. A comparison with the crystal structure of the receptor RNA bound to its tetraloop shows that a conformational change has to occur upon tetraloop binding, which is in good agreement with previous biochemical data. A model for an alternative binding site within the receptor is proposed based on the NMR structure, phylogenetic data and previous crystallographic structures of tetraloop interactions.
 ==About this Structure==
-TLR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TLR OCA].
+TLR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLR OCA].
 ==Reference==
 Solution structure of a GAAA tetraloop receptor RNA., Butcher SE, Dieckmann T, Feigon J, EMBO J. 1997 Dec 15;16(24):7490-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9405377 9405377]
 [[Category: Protein complex]]
-[[Category: Butcher, S.E.]]
+[[Category: Butcher, S E.]]
 [[Category: Dieckmann, T.]]
 [[Category: Feigon, J.]]
@@ Line 20: / Line 20: @@
 [[Category: tetraloop receptor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:23:14 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:14:54 2008''