1d53: Difference between revisions

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[[Image:1d53.gif|left|200px]]<br /><applet load="1d53" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1d53.gif|left|200px]]<br /><applet load="1d53" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1d53, resolution 1.500Å" />

'''CRYSTAL STRUCTURE AT 1.5 ANGSTROMS RESOLUTION OF D(CGCICICG), AN OCTANUCLEOTIDE CONTAINING INOSINE, AND ITS COMPARISON WITH D(CGCG) AND D(CGCGCG) STRUCTURES'''<br />

==Overview==

The octadeoxyribonucleotide d(CGCICICG) has been crystallized in space, group P(6)5(22) with unit cell dimensions of a = b = 31.0 A and c = 43.7, A, and X-ray diffraction data have been collected to 1.5-A resolution., Precession photographs and the self-Patterson function indicate that 12, base pairs of Z-conformation DNA stack along the c-axis, and the double, helices pack in a hexagonal array similar to that seen in other crystals, of Z-DNA. The structure has been solved by both Patterson deconvolution, and molecular replacement methods and refined in space group P(6)5 to an R, factor of 0.225 using 2503 unique reflections greater than 3.0 sigma (F)., Comparison of the molecules within the hexagonal lattice with highly, refined crystal structures of other Z-DNA reveals only minor, conformational differences, most notably in the pucker of the deoxyribose, of the purine residues. The DNA has multiple occupancy of C:I and C:G base, pairs, and C:I base pairs adopt a conformation similar to that of C:G base, pairs.

The octadeoxyribonucleotide d(CGCICICG) has been crystallized in space group P(6)5(22) with unit cell dimensions of a = b = 31.0 A and c = 43.7 A, and X-ray diffraction data have been collected to 1.5-A resolution. Precession photographs and the self-Patterson function indicate that 12 base pairs of Z-conformation DNA stack along the c-axis, and the double helices pack in a hexagonal array similar to that seen in other crystals of Z-DNA. The structure has been solved by both Patterson deconvolution and molecular replacement methods and refined in space group P(6)5 to an R factor of 0.225 using 2503 unique reflections greater than 3.0 sigma (F). Comparison of the molecules within the hexagonal lattice with highly refined crystal structures of other Z-DNA reveals only minor conformational differences, most notably in the pucker of the deoxyribose of the purine residues. The DNA has multiple occupancy of C:I and C:G base pairs, and C:I base pairs adopt a conformation similar to that of C:G base pairs.

==About this Structure==

1D53 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1D53 OCA].

1D53 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D53 OCA].

==Reference==

Crystal structure at 1.5-A resolution of d(CGCICICG), an octanucleotide containing inosine, and its comparison with d(CGCG) and d(CGCGCG) structures., Kumar VD, Harrison RW, Andrews LC, Weber IT, Biochemistry. 1992 Feb 11;31(5):1541-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1737011 1737011]

[[Category: Protein complex]]

[[Category: Andrews, L.C.]]

[[Category: Andrews, L C.]]

[[Category: Harrison, R.W.]]

[[Category: Harrison, R W.]]

[[Category: Kumar, V.D.]]

[[Category: Kumar, V D.]]

[[Category: Weber, I.T.]]

[[Category: Weber, I T.]]

[[Category: double helix]]

[[Category: z-dna]]

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@@ Line 1: / Line 1: @@
-[[Image:1d53.gif|left|200px]]<br /><applet load="1d53" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1d53.gif|left|200px]]<br /><applet load="1d53" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1d53, resolution 1.500&Aring;" />
 '''CRYSTAL STRUCTURE AT 1.5 ANGSTROMS RESOLUTION OF D(CGCICICG), AN OCTANUCLEOTIDE CONTAINING INOSINE, AND ITS COMPARISON WITH D(CGCG) AND D(CGCGCG) STRUCTURES'''<br />
 ==Overview==
-The octadeoxyribonucleotide d(CGCICICG) has been crystallized in space, group P(6)5(22) with unit cell dimensions of a = b = 31.0 A and c = 43.7, A, and X-ray diffraction data have been collected to 1.5-A resolution., Precession photographs and the self-Patterson function indicate that 12, base pairs of Z-conformation DNA stack along the c-axis, and the double, helices pack in a hexagonal array similar to that seen in other crystals, of Z-DNA. The structure has been solved by both Patterson deconvolution, and molecular replacement methods and refined in space group P(6)5 to an R, factor of 0.225 using 2503 unique reflections greater than 3.0 sigma (F)., Comparison of the molecules within the hexagonal lattice with highly, refined crystal structures of other Z-DNA reveals only minor, conformational differences, most notably in the pucker of the deoxyribose, of the purine residues. The DNA has multiple occupancy of C:I and C:G base, pairs, and C:I base pairs adopt a conformation similar to that of C:G base, pairs.
+The octadeoxyribonucleotide d(CGCICICG) has been crystallized in space group P(6)5(22) with unit cell dimensions of a = b = 31.0 A and c = 43.7 A, and X-ray diffraction data have been collected to 1.5-A resolution. Precession photographs and the self-Patterson function indicate that 12 base pairs of Z-conformation DNA stack along the c-axis, and the double helices pack in a hexagonal array similar to that seen in other crystals of Z-DNA. The structure has been solved by both Patterson deconvolution and molecular replacement methods and refined in space group P(6)5 to an R factor of 0.225 using 2503 unique reflections greater than 3.0 sigma (F). Comparison of the molecules within the hexagonal lattice with highly refined crystal structures of other Z-DNA reveals only minor conformational differences, most notably in the pucker of the deoxyribose of the purine residues. The DNA has multiple occupancy of C:I and C:G base pairs, and C:I base pairs adopt a conformation similar to that of C:G base pairs.
 ==About this Structure==
-D53 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D53 OCA].
+D53 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D53 OCA].
 ==Reference==
 Crystal structure at 1.5-A resolution of d(CGCICICG), an octanucleotide containing inosine, and its comparison with d(CGCG) and d(CGCGCG) structures., Kumar VD, Harrison RW, Andrews LC, Weber IT, Biochemistry. 1992 Feb 11;31(5):1541-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1737011 1737011]
 [[Category: Protein complex]]
-[[Category: Andrews, L.C.]]
+[[Category: Andrews, L C.]]
-[[Category: Harrison, R.W.]]
+[[Category: Harrison, R W.]]
-[[Category: Kumar, V.D.]]
+[[Category: Kumar, V D.]]
-[[Category: Weber, I.T.]]
+[[Category: Weber, I T.]]
 [[Category: double helix]]
 [[Category: z-dna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:30:14 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:02 2008''