1d64: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="1d64" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d64, resolution 2.100Å" /> '''CRYSTAL STRUCTURE O... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1d64.gif|left|200px]]<br /><applet load="1d64" size=" | [[Image:1d64.gif|left|200px]]<br /><applet load="1d64" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1d64, resolution 2.100Å" /> | caption="1d64, resolution 2.100Å" /> | ||
'''CRYSTAL STRUCTURE OF A PENTAMIDINE-OLIGONUCLEOTIDE COMPLEX: IMPLICATIONS FOR DNA-BINDING PROPERTIES'''<br /> | '''CRYSTAL STRUCTURE OF A PENTAMIDINE-OLIGONUCLEOTIDE COMPLEX: IMPLICATIONS FOR DNA-BINDING PROPERTIES'''<br /> | ||
==Overview== | ==Overview== | ||
The crystal structure of the complex formed between the dodecanucleotide | The crystal structure of the complex formed between the dodecanucleotide d(CGCGAATTCGCG)2 and the drug pentamidine, which is active against the Pneumocystis carinii pathogen in AIDS patients, has been determined to a resolution of 2.1 A and an R-factor of 19.4%. Analysis of the structure has shown the drug to be bound in the 5'-AATT minor groove region of the duplex, with the amidinium groups H-bonded to adenine N3 atoms in an interstrand manner. The drug molecule adopts an extended conformation, and the immediate binding site spans four base pairs. Structural details of the drug-DNA interactions are discussed, and comparison is made with the dodecamer complex of the structurally similar berenil ligand. | ||
==About this Structure== | ==About this Structure== | ||
1D64 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with PNT as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1D64 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=PNT:'>PNT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D64 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of a pentamidine-oligonucleotide complex: implications for DNA-binding properties., Edwards KJ, Jenkins TC, Neidle S, Biochemistry. 1992 Aug 11;31(31):7104-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1643044 1643044] | Crystal structure of a pentamidine-oligonucleotide complex: implications for DNA-binding properties., Edwards KJ, Jenkins TC, Neidle S, Biochemistry. 1992 Aug 11;31(31):7104-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1643044 1643044] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Edwards, K | [[Category: Edwards, K J.]] | ||
[[Category: Jenkins, T | [[Category: Jenkins, T C.]] | ||
[[Category: Neidle, S.]] | [[Category: Neidle, S.]] | ||
[[Category: PNT]] | [[Category: PNT]] | ||
| Line 20: | Line 20: | ||
[[Category: double helix]] | [[Category: double helix]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:23 2008'' | ||
