Isoelectric point: Difference between revisions
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==Alternative using Protein Calculator== | ==Alternative to calculate Isolelectic point using Protein Calculator== | ||
*In Proteopedia, find the page for your structure named for PDB file code and click on OCA under the structure. | *In Proteopedia, find the page for your structure named for PDB file code and click on OCA under the structure. | ||
* At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom). | * At your PDB code in OCA, scroll down to Sequence-derived information (near the bottom). | ||
*Click on the link for the one-letter amino acid sequence for one chain. | *Click on the link for the one-letter amino acid sequence for one chain. | ||
*Copy the sequence and paste it into the large box at the [http://www.scripps.edu/~cdputnam/protcalc.html Protein Calculator]. | *Copy the sequence and paste it into the large box at the [http://www.scripps.edu/~cdputnam/protcalc.html Protein Calculator]. | ||
*Check the first box under Charge at the right, and click Submit Query. | *Check the first box under Charge at the right, and click Submit Query. | ||
==Content Attribution== | ==Content Attribution== | ||
The first method was adapted from the [http://www.umass.edu/microbio/chime/pe_beta/pe/protexpl/igloss.htm Glossary] that accompanies [http://www.umass.edu/microbio/chime/pe_beta/pe/protexpl/frntdoo2.htm Eric Martz's Protein Explorer]. The second was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class sylabus taught by Eric Martz]. | The first method was adapted from the [http://www.umass.edu/microbio/chime/pe_beta/pe/protexpl/igloss.htm Glossary] that accompanies [http://www.umass.edu/microbio/chime/pe_beta/pe/protexpl/frntdoo2.htm Eric Martz's Protein Explorer]. The second was adapted from [http://www.umass.edu/molvis/workshop/osaka09m.htm a class sylabus taught by Eric Martz]. | ||
