1xou: Difference between revisions

Line 1:

[[Image:1xou.jpg|left|200px]]<br /><applet load="1xou" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1xou.jpg|left|200px]]<br /><applet load="1xou" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1xou, resolution 2.80Å" />

'''Crystal structure of the CesA-EspA complex'''<br />

==Overview==

The type III secretion system (TTSS) mediates the specific translocation, of bacterial proteins into the cytoplasm of eukaryotic cells, a process, essential for the virulence of many Gram-negative pathogens. The, enteropathogenic Escherichia coli TTSS protein EspA forms a hollow, extracellular filament believed to be a molecular conduit for type III, protein translocation. Structural analysis of EspA has been hampered by, its polymeric nature. We show that EspA alone is sufficient to form, filamentous structures in the absence of other pathogenicity, island-encoded proteins. CesA is the recently proposed chaperone of EspA, and we demonstrate that CesA traps EspA in a monomeric state and inhibits, its polymerization. Crystallographic analysis of the heterodimeric, CesA-EspA complex at a resolution of 2.8 A reveals that EspA contains two, long a-helices, which are involved in extensive coiled-coil interactions, with CesA.

The type III secretion system (TTSS) mediates the specific translocation of bacterial proteins into the cytoplasm of eukaryotic cells, a process essential for the virulence of many Gram-negative pathogens. The enteropathogenic Escherichia coli TTSS protein EspA forms a hollow extracellular filament believed to be a molecular conduit for type III protein translocation. Structural analysis of EspA has been hampered by its polymeric nature. We show that EspA alone is sufficient to form filamentous structures in the absence of other pathogenicity island-encoded proteins. CesA is the recently proposed chaperone of EspA, and we demonstrate that CesA traps EspA in a monomeric state and inhibits its polymerization. Crystallographic analysis of the heterodimeric CesA-EspA complex at a resolution of 2.8 A reveals that EspA contains two long a-helices, which are involved in extensive coiled-coil interactions with CesA.

==About this Structure==

1XOU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1XOU OCA].

1XOU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XOU OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Protein complex]]

[[Category: Finlay, B.B.]]

[[Category: Finlay, B B.]]

[[Category: Strynadka, N.C.J.]]

[[Category: Strynadka, N C.J.]]

[[Category: Yip, C.K.]]

[[Category: Yip, C K.]]

[[Category: coiled coil]]

[[Category: helix bundle]]

[[Category: heterodimer]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 02~~:36:~~32 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:57:04 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1xou.jpg|left|200px]]<br /><applet load="1xou" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1xou.jpg|left|200px]]<br /><applet load="1xou" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1xou, resolution 2.80&Aring;" />
 '''Crystal structure of the CesA-EspA complex'''<br />
 ==Overview==
-The type III secretion system (TTSS) mediates the specific translocation, of bacterial proteins into the cytoplasm of eukaryotic cells, a process, essential for the virulence of many Gram-negative pathogens. The, enteropathogenic Escherichia coli TTSS protein EspA forms a hollow, extracellular filament believed to be a molecular conduit for type III, protein translocation. Structural analysis of EspA has been hampered by, its polymeric nature. We show that EspA alone is sufficient to form, filamentous structures in the absence of other pathogenicity, island-encoded proteins. CesA is the recently proposed chaperone of EspA, and we demonstrate that CesA traps EspA in a monomeric state and inhibits, its polymerization. Crystallographic analysis of the heterodimeric, CesA-EspA complex at a resolution of 2.8 A reveals that EspA contains two, long a-helices, which are involved in extensive coiled-coil interactions, with CesA.
+The type III secretion system (TTSS) mediates the specific translocation of bacterial proteins into the cytoplasm of eukaryotic cells, a process essential for the virulence of many Gram-negative pathogens. The enteropathogenic Escherichia coli TTSS protein EspA forms a hollow extracellular filament believed to be a molecular conduit for type III protein translocation. Structural analysis of EspA has been hampered by its polymeric nature. We show that EspA alone is sufficient to form filamentous structures in the absence of other pathogenicity island-encoded proteins. CesA is the recently proposed chaperone of EspA, and we demonstrate that CesA traps EspA in a monomeric state and inhibits its polymerization. Crystallographic analysis of the heterodimeric CesA-EspA complex at a resolution of 2.8 A reveals that EspA contains two long a-helices, which are involved in extensive coiled-coil interactions with CesA.
 ==About this Structure==
-XOU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XOU OCA].
+XOU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XOU OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Protein complex]]
-[[Category: Finlay, B.B.]]
+[[Category: Finlay, B B.]]
-[[Category: Strynadka, N.C.J.]]
+[[Category: Strynadka, N C.J.]]
-[[Category: Yip, C.K.]]
+[[Category: Yip, C K.]]
 [[Category: coiled coil]]
 [[Category: helix bundle]]
 [[Category: heterodimer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:36:32 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:57:04 2008''