1l8f: Difference between revisions

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[[Image:1l8f.jpg|left|200px]]<br /><applet load="1l8f" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1l8f.jpg|left|200px]]<br /><applet load="1l8f" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1l8f, resolution 1.8Å" />

'''Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A'''<br />

==Overview==

The crystal structure of the 20K endoglucanase from the thermophilic, fungus Melanocarpus albomyces (Ma20k) has been determined. The structure, was refined to 1.8 A resolution using data obtained at 120 K. Ma20k, belongs to glycoside hydrolase family 45. The three-dimensional structures, of endoglucanase V (EGV) from the fungus Humicola insolens and of an, endoglucanase from H. grisea var. thermoidea have previously been, determined. The overall structure of Ma20k consists of a six-stranded, beta-barrel domain similar to that found previously in family 45, endoglucanases. The flexible loop between strands V and VI, which was, disordered in the uncomplexed structures of the Humicola endoglucanases, but was ordered in complexed structures of EGV, is found to be well, ordered in the native structure of Ma20k. The structure of Ma20k allows, comparison between thermophilic and mesophilic proteins of family 45 and, different principles for thermostability are discussed.

The crystal structure of the 20K endoglucanase from the thermophilic fungus Melanocarpus albomyces (Ma20k) has been determined. The structure was refined to 1.8 A resolution using data obtained at 120 K. Ma20k belongs to glycoside hydrolase family 45. The three-dimensional structures of endoglucanase V (EGV) from the fungus Humicola insolens and of an endoglucanase from H. grisea var. thermoidea have previously been determined. The overall structure of Ma20k consists of a six-stranded beta-barrel domain similar to that found previously in family 45 endoglucanases. The flexible loop between strands V and VI, which was disordered in the uncomplexed structures of the Humicola endoglucanases but was ordered in complexed structures of EGV, is found to be well ordered in the native structure of Ma20k. The structure of Ma20k allows comparison between thermophilic and mesophilic proteins of family 45 and different principles for thermostability are discussed.

==About this Structure==

1L8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1L8F OCA].

1L8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L8F OCA].

==Reference==

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[[Category: thermophilic]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 02~~:38:~~13 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:42:26 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1l8f.jpg|left|200px]]<br /><applet load="1l8f" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1l8f.jpg|left|200px]]<br /><applet load="1l8f" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1l8f, resolution 1.8&Aring;" />
 '''Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A'''<br />
 ==Overview==
-The crystal structure of the 20K endoglucanase from the thermophilic, fungus Melanocarpus albomyces (Ma20k) has been determined. The structure, was refined to 1.8 A resolution using data obtained at 120 K. Ma20k, belongs to glycoside hydrolase family 45. The three-dimensional structures, of endoglucanase V (EGV) from the fungus Humicola insolens and of an, endoglucanase from H. grisea var. thermoidea have previously been, determined. The overall structure of Ma20k consists of a six-stranded, beta-barrel domain similar to that found previously in family 45, endoglucanases. The flexible loop between strands V and VI, which was, disordered in the uncomplexed structures of the Humicola endoglucanases, but was ordered in complexed structures of EGV, is found to be well, ordered in the native structure of Ma20k. The structure of Ma20k allows, comparison between thermophilic and mesophilic proteins of family 45 and, different principles for thermostability are discussed.
+The crystal structure of the 20K endoglucanase from the thermophilic fungus Melanocarpus albomyces (Ma20k) has been determined. The structure was refined to 1.8 A resolution using data obtained at 120 K. Ma20k belongs to glycoside hydrolase family 45. The three-dimensional structures of endoglucanase V (EGV) from the fungus Humicola insolens and of an endoglucanase from H. grisea var. thermoidea have previously been determined. The overall structure of Ma20k consists of a six-stranded beta-barrel domain similar to that found previously in family 45 endoglucanases. The flexible loop between strands V and VI, which was disordered in the uncomplexed structures of the Humicola endoglucanases but was ordered in complexed structures of EGV, is found to be well ordered in the native structure of Ma20k. The structure of Ma20k allows comparison between thermophilic and mesophilic proteins of family 45 and different principles for thermostability are discussed.
 ==About this Structure==
-L8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1L8F OCA].
+L8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L8F OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: thermophilic]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:38:13 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:42:26 2008''