1pnn: Difference between revisions

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[[Image:1pnn.gif|left|200px]]<br /><applet load="1pnn" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1pnn.gif|left|200px]]<br /><applet load="1pnn" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1pnn, resolution 2.5Å" />

'''PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA'''<br />

==Overview==

The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid, structures. The triplex consists of one polypurine DNA strand complexed to, a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully, designed to promote Watson-Crick and Hoogsteen base pairing. The P-form, helix is underwound, with a base tilt similar to B-form DNA. The bases are, displaced from the helix axis even more than in A-form DNA. Hydrogen bonds, between the DNA backbone and the Hoogsteen PNA backbone explain the, observation that polypyrimidine PNA sequences form highly stable 2:1, PNA-DNA complexes. This structure expands the number of known stable, helical forms that nucleic acids can adopt.

The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid structures. The triplex consists of one polypurine DNA strand complexed to a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully designed to promote Watson-Crick and Hoogsteen base pairing. The P-form helix is underwound, with a base tilt similar to B-form DNA. The bases are displaced from the helix axis even more than in A-form DNA. Hydrogen bonds between the DNA backbone and the Hoogsteen PNA backbone explain the observation that polypyrimidine PNA sequences form highly stable 2:1 PNA-DNA complexes. This structure expands the number of known stable helical forms that nucleic acids can adopt.

==About this Structure==

1PNN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PNN OCA].

1PNN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNN OCA].

==Reference==

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[[Category: Protein complex]]

[[Category: Betts, L.]]

[[Category: Veal, J.M.]]

[[Category: Veal, J M.]]

[[Category: hairpin pna:dna triplex]]

[[Category: triplex watson-crick hoogsteen]]

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@@ Line 1: / Line 1: @@
-[[Image:1pnn.gif|left|200px]]<br /><applet load="1pnn" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pnn.gif|left|200px]]<br /><applet load="1pnn" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pnn, resolution 2.5&Aring;" />
 '''PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA'''<br />
 ==Overview==
-The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid, structures. The triplex consists of one polypurine DNA strand complexed to, a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully, designed to promote Watson-Crick and Hoogsteen base pairing. The P-form, helix is underwound, with a base tilt similar to B-form DNA. The bases are, displaced from the helix axis even more than in A-form DNA. Hydrogen bonds, between the DNA backbone and the Hoogsteen PNA backbone explain the, observation that polypyrimidine PNA sequences form highly stable 2:1, PNA-DNA complexes. This structure expands the number of known stable, helical forms that nucleic acids can adopt.
+The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid structures. The triplex consists of one polypurine DNA strand complexed to a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully designed to promote Watson-Crick and Hoogsteen base pairing. The P-form helix is underwound, with a base tilt similar to B-form DNA. The bases are displaced from the helix axis even more than in A-form DNA. Hydrogen bonds between the DNA backbone and the Hoogsteen PNA backbone explain the observation that polypyrimidine PNA sequences form highly stable 2:1 PNA-DNA complexes. This structure expands the number of known stable helical forms that nucleic acids can adopt.
 ==About this Structure==
-PNN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PNN OCA].
+PNN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNN OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Betts, L.]]
-[[Category: Veal, J.M.]]
+[[Category: Veal, J M.]]
 [[Category: hairpin pna:dna triplex]]
 [[Category: triplex watson-crick hoogsteen]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:44:45 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:37 2008''