1d91: Difference between revisions

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[[Image:1d91.gif|left|200px]]<br /><applet load="1d91" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1d91.gif|left|200px]]<br /><applet load="1d91" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1d91, resolution 2.100Å" />

'''G.T BASE PAIRS IN A DNA HELIX. THE CRYSTAL STRUCTURE OF D(G-G-G-G-T-C-C-C)'''<br />

==Overview==

The synthetic deoxyoctanucleotide d(G-G-G-G-T-C-C-C) crystallizes as an, A-type DNA double helix containing two adjacent G . T base-pair, mismatches. The structure has been refined to an R-factor of 14% at 2.1 A, resolution with 104 solvent molecules located. The two G . T mismatches, adopt the "wobble" form of base-pairing. The mismatched bases are linked, by a network of water molecules interacting with the exposed functional, groups in both the major and minor grooves. The presence of two mispaired, bases in the octamer has surprisingly little effect on the global, structure of the helix or the backbone and glycosidic torsional angles., Base stacking around the mismatch is perturbed, but the central G-T step, shows particularly good base overlap, which may contribute to the, relatively high stability of this oligomer.

The synthetic deoxyoctanucleotide d(G-G-G-G-T-C-C-C) crystallizes as an A-type DNA double helix containing two adjacent G . T base-pair mismatches. The structure has been refined to an R-factor of 14% at 2.1 A resolution with 104 solvent molecules located. The two G . T mismatches adopt the "wobble" form of base-pairing. The mismatched bases are linked by a network of water molecules interacting with the exposed functional groups in both the major and minor grooves. The presence of two mispaired bases in the octamer has surprisingly little effect on the global structure of the helix or the backbone and glycosidic torsional angles. Base stacking around the mismatch is perturbed, but the central G-T step shows particularly good base overlap, which may contribute to the relatively high stability of this oligomer.

==About this Structure==

1D91 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1D91 OCA].

1D91 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D91 OCA].

==Reference==

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[[Category: mismatched]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 02~~:46:~~59 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:17 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1d91.gif|left|200px]]<br /><applet load="1d91" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1d91.gif|left|200px]]<br /><applet load="1d91" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1d91, resolution 2.100&Aring;" />
 '''G.T BASE PAIRS IN A DNA HELIX. THE CRYSTAL STRUCTURE OF D(G-G-G-G-T-C-C-C)'''<br />
 ==Overview==
-The synthetic deoxyoctanucleotide d(G-G-G-G-T-C-C-C) crystallizes as an, A-type DNA double helix containing two adjacent G . T base-pair, mismatches. The structure has been refined to an R-factor of 14% at 2.1 A, resolution with 104 solvent molecules located. The two G . T mismatches, adopt the "wobble" form of base-pairing. The mismatched bases are linked, by a network of water molecules interacting with the exposed functional, groups in both the major and minor grooves. The presence of two mispaired, bases in the octamer has surprisingly little effect on the global, structure of the helix or the backbone and glycosidic torsional angles., Base stacking around the mismatch is perturbed, but the central G-T step, shows particularly good base overlap, which may contribute to the, relatively high stability of this oligomer.
+The synthetic deoxyoctanucleotide d(G-G-G-G-T-C-C-C) crystallizes as an A-type DNA double helix containing two adjacent G . T base-pair mismatches. The structure has been refined to an R-factor of 14% at 2.1 A resolution with 104 solvent molecules located. The two G . T mismatches adopt the "wobble" form of base-pairing. The mismatched bases are linked by a network of water molecules interacting with the exposed functional groups in both the major and minor grooves. The presence of two mispaired bases in the octamer has surprisingly little effect on the global structure of the helix or the backbone and glycosidic torsional angles. Base stacking around the mismatch is perturbed, but the central G-T step shows particularly good base overlap, which may contribute to the relatively high stability of this oligomer.
 ==About this Structure==
-D91 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D91 OCA].
+D91 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D91 OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: mismatched]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:46:59 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:17 2008''