1pp8: Difference between revisions

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[[Image:1pp8.gif|left|200px]]<br /><applet load="1pp8" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1pp8.gif|left|200px]]<br /><applet load="1pp8" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1pp8, resolution 3.05Å" />

'''crystal structure of the T. vaginalis IBP39 Initiator binding domain (IBD) bound to the alpha-SCS Inr element'''<br />

==Overview==

Transcription start site selection in eukaryotes is mediated through, combinations of the TATA, initiator (Inr), and downstream promoter, elements (DPE). In Trichomonas vaginalis, a parabasalian flagellate, thought to represent an ancient eukaryote lineage, the Inr appears to be, solely responsible for start site selection and is recognized by the, initiator binding protein 39 kDa (IBP39). IBP39 contains an N-terminal Inr, binding domain (IBD) connected via a flexible linker to a C-terminal, domain (C domain). Here we present crystal structures of the apoIBD and, IBD-Inr complexes and the C domain. The IBD structures reveal a, winged-helix motif with prokaryotic and eukaryotic features and a scaffold, similar to that of ETS-family proteins. The C domain structure and, biochemical studies indicate that it interacts with the T. vaginalis RNAP, II large subunit C-terminal domain. These data suggest that binding of, IBP39 to the Inr directly recruits RNAP II and in this way initiates, transcription.

Transcription start site selection in eukaryotes is mediated through combinations of the TATA, initiator (Inr), and downstream promoter elements (DPE). In Trichomonas vaginalis, a parabasalian flagellate thought to represent an ancient eukaryote lineage, the Inr appears to be solely responsible for start site selection and is recognized by the initiator binding protein 39 kDa (IBP39). IBP39 contains an N-terminal Inr binding domain (IBD) connected via a flexible linker to a C-terminal domain (C domain). Here we present crystal structures of the apoIBD and IBD-Inr complexes and the C domain. The IBD structures reveal a winged-helix motif with prokaryotic and eukaryotic features and a scaffold similar to that of ETS-family proteins. The C domain structure and biochemical studies indicate that it interacts with the T. vaginalis RNAP II large subunit C-terminal domain. These data suggest that binding of IBP39 to the Inr directly recruits RNAP II and in this way initiates transcription.

==About this Structure==

1PP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Trichomonas_vaginalis Trichomonas vaginalis] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1PP8 OCA].

1PP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Trichomonas_vaginalis Trichomonas vaginalis] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PP8 OCA].

==Reference==

Line 13:

[[Category: Single protein]]

[[Category: Trichomonas vaginalis]]

[[Category: Johnson, P.J.]]

[[Category: Johnson, P J.]]

[[Category: Lau, A.O.T.]]

[[Category: Lau, A O.T.]]

[[Category: Schumacher, M.A.]]

[[Category: Schumacher, M A.]]

[[Category: SO4]]

[[Category: core promoter]]

Line 24:

[[Category: transcription]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 02~~:49:~~57 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:31:05 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1pp8.gif|left|200px]]<br /><applet load="1pp8" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1pp8.gif|left|200px]]<br /><applet load="1pp8" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1pp8, resolution 3.05&Aring;" />
 '''crystal structure of the T. vaginalis IBP39 Initiator binding domain (IBD) bound to the alpha-SCS Inr element'''<br />
 ==Overview==
-Transcription start site selection in eukaryotes is mediated through, combinations of the TATA, initiator (Inr), and downstream promoter, elements (DPE). In Trichomonas vaginalis, a parabasalian flagellate, thought to represent an ancient eukaryote lineage, the Inr appears to be, solely responsible for start site selection and is recognized by the, initiator binding protein 39 kDa (IBP39). IBP39 contains an N-terminal Inr, binding domain (IBD) connected via a flexible linker to a C-terminal, domain (C domain). Here we present crystal structures of the apoIBD and, IBD-Inr complexes and the C domain. The IBD structures reveal a, winged-helix motif with prokaryotic and eukaryotic features and a scaffold, similar to that of ETS-family proteins. The C domain structure and, biochemical studies indicate that it interacts with the T. vaginalis RNAP, II large subunit C-terminal domain. These data suggest that binding of, IBP39 to the Inr directly recruits RNAP II and in this way initiates, transcription.
+Transcription start site selection in eukaryotes is mediated through combinations of the TATA, initiator (Inr), and downstream promoter elements (DPE). In Trichomonas vaginalis, a parabasalian flagellate thought to represent an ancient eukaryote lineage, the Inr appears to be solely responsible for start site selection and is recognized by the initiator binding protein 39 kDa (IBP39). IBP39 contains an N-terminal Inr binding domain (IBD) connected via a flexible linker to a C-terminal domain (C domain). Here we present crystal structures of the apoIBD and IBD-Inr complexes and the C domain. The IBD structures reveal a winged-helix motif with prokaryotic and eukaryotic features and a scaffold similar to that of ETS-family proteins. The C domain structure and biochemical studies indicate that it interacts with the T. vaginalis RNAP II large subunit C-terminal domain. These data suggest that binding of IBP39 to the Inr directly recruits RNAP II and in this way initiates transcription.
 ==About this Structure==
-PP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Trichomonas_vaginalis Trichomonas vaginalis] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PP8 OCA].
+PP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Trichomonas_vaginalis Trichomonas vaginalis] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PP8 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Trichomonas vaginalis]]
-[[Category: Johnson, P.J.]]
+[[Category: Johnson, P J.]]
-[[Category: Lau, A.O.T.]]
+[[Category: Lau, A O.T.]]
-[[Category: Schumacher, M.A.]]
+[[Category: Schumacher, M A.]]
 [[Category: SO4]]
 [[Category: core promoter]]
@@ Line 24: / Line 24: @@
 [[Category: transcription]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:49:57 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:31:05 2008''