1da2: Difference between revisions

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[[Image:1da2.gif|left|200px]]<br /><applet load="1da2" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1da2.gif|left|200px]]<br /><applet load="1da2" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1da2, resolution 1.700Å" />

'''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO4CG): N4-METHOXYCYTOSINE/GUANINE BASE-PAIRS IN Z-DNA'''<br />

==Overview==

The base analogue N4-methoxycytosine (mo4C) is ambivalent in its, hydrogen-bonding potential, since it forms stable base-pairs with both, adenine and guanine in oligomer duplexes. To investigate the base-pair, geometry, the structure of d(CGCGmo4CG) has been determined by, single-crystal X-ray diffraction techniques. The d(CGCGmo4CG)2, crystallized in a left-handed double helical structure (Z-type)., Refinement using 2559 reflections between 10 and 1.7 A converged with a, final R = 0.181 (Rw = 0.130) including 68 solvent molecules. The, orthorhombic crystals are in the space group P2(1)2(1)2(1), with cell, dimensions a = 18.17 A, b = 30.36 A, c = 43.93 A. The mo4C.G base-pair is, of the wobble type, with mo4C in the imino form, and the methoxy group in, the syn configuration.

The base analogue N4-methoxycytosine (mo4C) is ambivalent in its hydrogen-bonding potential, since it forms stable base-pairs with both adenine and guanine in oligomer duplexes. To investigate the base-pair geometry, the structure of d(CGCGmo4CG) has been determined by single-crystal X-ray diffraction techniques. The d(CGCGmo4CG)2 crystallized in a left-handed double helical structure (Z-type). Refinement using 2559 reflections between 10 and 1.7 A converged with a final R = 0.181 (Rw = 0.130) including 68 solvent molecules. The orthorhombic crystals are in the space group P2(1)2(1)2(1), with cell dimensions a = 18.17 A, b = 30.36 A, c = 43.93 A. The mo4C.G base-pair is of the wobble type, with mo4C in the imino form, and the methoxy group in the syn configuration.

==About this Structure==

1DA2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1DA2 OCA].

1DA2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DA2 OCA].

==Reference==

Molecular and crystal structure of d(CGCGmo4CG): N4-methoxycytosine.guanine base-pairs in Z-DNA., Van Meervelt L, Moore MH, Lin PK, Brown DM, Kennard O, J Mol Biol. 1990 Dec 5;216(3):773-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2258939 2258939]

[[Category: Protein complex]]

[[Category: Brown, D.M.]]

[[Category: Brown, D M.]]

[[Category: Kennard, O.]]

[[Category: Lin, P.K.T.]]

[[Category: Lin, P K.T.]]

[[Category: Meervelt, L.Van.]]

[[Category: Meervelt, L Van.]]

[[Category: Moore, M.H.]]

[[Category: Moore, M H.]]

[[Category: double helix]]

[[Category: modified]]

[[Category: z-dna]]

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@@ Line 1: / Line 1: @@
-[[Image:1da2.gif|left|200px]]<br /><applet load="1da2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1da2.gif|left|200px]]<br /><applet load="1da2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1da2, resolution 1.700&Aring;" />
 '''MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO4CG): N4-METHOXYCYTOSINE/GUANINE BASE-PAIRS IN Z-DNA'''<br />
 ==Overview==
-The base analogue N4-methoxycytosine (mo4C) is ambivalent in its, hydrogen-bonding potential, since it forms stable base-pairs with both, adenine and guanine in oligomer duplexes. To investigate the base-pair, geometry, the structure of d(CGCGmo4CG) has been determined by, single-crystal X-ray diffraction techniques. The d(CGCGmo4CG)2, crystallized in a left-handed double helical structure (Z-type)., Refinement using 2559 reflections between 10 and 1.7 A converged with a, final R = 0.181 (Rw = 0.130) including 68 solvent molecules. The, orthorhombic crystals are in the space group P2(1)2(1)2(1), with cell, dimensions a = 18.17 A, b = 30.36 A, c = 43.93 A. The mo4C.G base-pair is, of the wobble type, with mo4C in the imino form, and the methoxy group in, the syn configuration.
+The base analogue N4-methoxycytosine (mo4C) is ambivalent in its hydrogen-bonding potential, since it forms stable base-pairs with both adenine and guanine in oligomer duplexes. To investigate the base-pair geometry, the structure of d(CGCGmo4CG) has been determined by single-crystal X-ray diffraction techniques. The d(CGCGmo4CG)2 crystallized in a left-handed double helical structure (Z-type). Refinement using 2559 reflections between 10 and 1.7 A converged with a final R = 0.181 (Rw = 0.130) including 68 solvent molecules. The orthorhombic crystals are in the space group P2(1)2(1)2(1), with cell dimensions a = 18.17 A, b = 30.36 A, c = 43.93 A. The mo4C.G base-pair is of the wobble type, with mo4C in the imino form, and the methoxy group in the syn configuration.
 ==About this Structure==
-DA2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DA2 OCA].
+DA2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DA2 OCA].
 ==Reference==
 Molecular and crystal structure of d(CGCGmo4CG): N4-methoxycytosine.guanine base-pairs in Z-DNA., Van Meervelt L, Moore MH, Lin PK, Brown DM, Kennard O, J Mol Biol. 1990 Dec 5;216(3):773-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2258939 2258939]
 [[Category: Protein complex]]
-[[Category: Brown, D.M.]]
+[[Category: Brown, D M.]]
 [[Category: Kennard, O.]]
-[[Category: Lin, P.K.T.]]
+[[Category: Lin, P K.T.]]
-[[Category: Meervelt, L.Van.]]
+[[Category: Meervelt, L Van.]]
-[[Category: Moore, M.H.]]
+[[Category: Moore, M H.]]
 [[Category: double helix]]
 [[Category: modified]]
 [[Category: z-dna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:51:43 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:31 2008''