1xt8: Difference between revisions
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New page: left|200px<br /><applet load="1xt8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xt8, resolution 2.00Å" /> '''Crystal Structure of... |
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[[Image:1xt8.gif|left|200px]]<br /><applet load="1xt8" size=" | [[Image:1xt8.gif|left|200px]]<br /><applet load="1xt8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1xt8, resolution 2.00Å" /> | caption="1xt8, resolution 2.00Å" /> | ||
'''Crystal Structure of Cysteine-Binding Protein from Campylobacter jejuni at 2.0 A Resolution'''<br /> | '''Crystal Structure of Cysteine-Binding Protein from Campylobacter jejuni at 2.0 A Resolution'''<br /> | ||
==Overview== | ==Overview== | ||
Campylobacter jejuni is a Gram-negative food-borne pathogen associated | Campylobacter jejuni is a Gram-negative food-borne pathogen associated with gastroenteritis in humans as well as cases of the autoimmune disease Guillain-Barre syndrome. C. jejuni is asaccharolytic because it lacks an active glycolytic pathway for the use of sugars as a carbon source. This suggests an increased reliance on amino acids as nutrients and indeed the genome sequence of this organism indicates the presence of a number of amino acid uptake systems. Cj0982, also known as CjaA, is a putative extracytoplasmic solute receptor for one such uptake system as well as a major surface antigen and vaccine candidate. The crystal structure of Cj0982 reveals a two-domain protein with density in the enclosed cavity between the domains that clearly defines the presence of a bound cysteine ligand. Fluorescence titration experiments were used to demonstrate that Cj0982 binds cysteine tightly and specifically with a K(d) of approximately 10(-7) M consistent with a role as a receptor for a high-affinity transporter. These data imply that Cj0982 is the binding protein component of an ABC-type cysteine transporter system and that cysteine uptake is important in the physiology of C. jejuni. | ||
==About this Structure== | ==About this Structure== | ||
1XT8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni] with CYS and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1XT8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni] with <scene name='pdbligand=CYS:'>CYS</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT8 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Blagova, E.]] | [[Category: Blagova, E.]] | ||
[[Category: Brannigan, J | [[Category: Brannigan, J A.]] | ||
[[Category: Fogg, M | [[Category: Fogg, M J.]] | ||
[[Category: Horler, R.]] | [[Category: Horler, R.]] | ||
[[Category: Levdikov, V | [[Category: Levdikov, V M.]] | ||
[[Category: Muller, A.]] | [[Category: Muller, A.]] | ||
[[Category: SPINE, Structural | [[Category: SPINE, Structural Proteomics in Europe.]] | ||
[[Category: Thomas, G | [[Category: Thomas, G H.]] | ||
[[Category: Wilkinson, A | [[Category: Wilkinson, A J.]] | ||
[[Category: Wilson, K | [[Category: Wilson, K S.]] | ||
[[Category: CYS]] | [[Category: CYS]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:25 2008'' | ||
