Sandbox122: Difference between revisions
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The FMN (Flavin MonoNucleotide) is the ligand which is responsible for the light absorption. A single molecule of FMN is bound non convalently in the interior of LOV2 domain. FMN is stabilized thanks to hydrogen bonds, Van der Waals and electrostatic interactions. For example, atoms R983 and R967(alpha C helix) create ionic bond with phosphate group of FMN. Q970,N965,N998,N1008(alpha A helix and beta-strand C,D and E) aminoacid make some | The FMN (Flavin MonoNucleotide) is the ligand which is responsible for the light absorption. A single molecule of FMN is bound non convalently in the interior of LOV2 domain. FMN is stabilized thanks to hydrogen bonds, Van der Waals and electrostatic interactions. For example, atoms R983 and R967(alpha C helix) create ionic bond with phosphate group of FMN. Q970,N965,N998,N1008(alpha A helix and beta-strand C,D and E) aminoacid make some electrostatic interaction which stabilize FMN. | ||
Blue light arrives on the only LOV2 cysteine residue(situate 4.2A from atom C(4a)) and induces formation of covalent cysteinyl-C(4a)adduct. (To explain that, refer to the scheme). This complex may interact directly with the kinase and regulate is activity. | Blue light arrives on the only LOV2 cysteine residue(situate 4.2A from atom C(4a)) and induces formation of covalent cysteinyl-C(4a)adduct. (To explain that, refer to the scheme). This complex may interact directly with the kinase and regulate is activity. | ||
Kinase will permit the autophosphorylation of phototropin. The rate of phosphorylatide phototropin acts to differential lateral gradients of auxin which is responsible of phototropism phenomenon. | Kinase will permit the autophosphorylation of phototropin. The rate of phosphorylatide phototropin acts to differential lateral gradients of auxin which is responsible of phototropism phenomenon. | ||
[[Image:1g28.jpg | thumb]] | [[Image:1g28.jpg | thumb]] | ||
[[Image:1G28.pdb_b.jpg | thumb]] | [[Image:1G28.pdb_b.jpg | thumb]] | ||
