1u26: Difference between revisions
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New page: left|200px<br /><applet load="1u26" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u26, resolution 2.5Å" /> '''Crystal structure of ... |
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[[Image:1u26.gif|left|200px]]<br /><applet load="1u26" size=" | [[Image:1u26.gif|left|200px]]<br /><applet load="1u26" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1u26, resolution 2.5Å" /> | caption="1u26, resolution 2.5Å" /> | ||
'''Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate'''<br /> | '''Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate'''<br /> | ||
==Overview== | ==Overview== | ||
Various inositide phosphatases participate in the regulation of inositol | Various inositide phosphatases participate in the regulation of inositol polyphosphate signaling molecules. Plant phytases are phosphatases that hydrolyze phytate to less-phosphorylated myo-inositol derivatives and phosphate. The phytase from Selenomonas ruminantium shares no sequence homology with other microbial phytases. Its crystal structure revealed a phytase fold of the dual-specificity phosphatase type. The active site is located near a conserved cysteine-containing (Cys241) P loop. We also solved two other crystal forms in which an inhibitor, myo-inositol hexasulfate, is cocrystallized with the enzyme. In the "standby" and the "inhibited" crystal forms, the inhibitor is bound, respectively, in a pocket slightly away from Cys241 and at the substrate binding site where the phosphate group to be hydrolyzed is held close to the -SH group of Cys241. Our structural and mutagenesis studies allow us to visualize the way in which the P loop-containing phytase attracts and hydrolyzes the substrate (phytate) sequentially. | ||
==About this Structure== | ==About this Structure== | ||
1U26 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium] with IHS as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/5-phytase 5-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.72 3.1.3.72] Full crystallographic information is available from [http:// | 1U26 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium] with <scene name='pdbligand=IHS:'>IHS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/5-phytase 5-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.72 3.1.3.72] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U26 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Selenomonas ruminantium]] | [[Category: Selenomonas ruminantium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Cheng, K | [[Category: Cheng, K J.]] | ||
[[Category: Chou, C | [[Category: Chou, C C.]] | ||
[[Category: Chu, H | [[Category: Chu, H M.]] | ||
[[Category: Guo, R | [[Category: Guo, R T.]] | ||
[[Category: Lai, H | [[Category: Lai, H L.]] | ||
[[Category: Lin, T | [[Category: Lin, T W.]] | ||
[[Category: Selinger, B | [[Category: Selinger, B L.]] | ||
[[Category: Shr, H | [[Category: Shr, H L.]] | ||
[[Category: Tang, T | [[Category: Tang, T Y.]] | ||
[[Category: Wang, A | [[Category: Wang, A H.J.]] | ||
[[Category: IHS]] | [[Category: IHS]] | ||
[[Category: p-loop]] | [[Category: p-loop]] | ||
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[[Category: ptp]] | [[Category: ptp]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:49 2008'' | ||
