1hwq: Difference between revisions

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[[Image:1hwq.gif|left|200px]]<br /><applet load="1hwq" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1hwq.gif|left|200px]]<br /><applet load="1hwq" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1hwq" />

'''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP'''<br />

==Overview==

The VS ribozyme is a 154 nucleotide sequence found in certain natural, strains of Neurospora. The RNA can be divided into a substrate and a, catalytic domain. Here we present the solution structure of the substrate, RNA that is cleaved in a trans reaction by the catalytic domain in the, presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix, capped by a flexible loop. The cleavage site bulge contains three, non-canonical base-pairs, including an A+.C pair with a protonated, adenine. This adenine (A622) is a pH controlled conformational switch that, opens up the internal loop at higher pH. The possible significance of this, switch for substrate recognition and cleavage is discussed.

The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed.

==About this Structure==

1HWQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA].

1HWQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA].

==Reference==

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[[Category: Protein complex]]

[[Category: Dieckmann, T.]]

[[Category: Flinders, J.C.]]

[[Category: Flinders, J C.]]

[[Category: a+c base pair]]

[[Category: protonated adenine]]

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[[Category: vs ribozyme]]

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@@ Line 1: / Line 1: @@
-[[Image:1hwq.gif|left|200px]]<br /><applet load="1hwq" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1hwq.gif|left|200px]]<br /><applet load="1hwq" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1hwq" />
 '''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP'''<br />
 ==Overview==
-The VS ribozyme is a 154 nucleotide sequence found in certain natural, strains of Neurospora. The RNA can be divided into a substrate and a, catalytic domain. Here we present the solution structure of the substrate, RNA that is cleaved in a trans reaction by the catalytic domain in the, presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix, capped by a flexible loop. The cleavage site bulge contains three, non-canonical base-pairs, including an A+.C pair with a protonated, adenine. This adenine (A622) is a pH controlled conformational switch that, opens up the internal loop at higher pH. The possible significance of this, switch for substrate recognition and cleavage is discussed.
+The VS ribozyme is a 154 nucleotide sequence found in certain natural strains of Neurospora. The RNA can be divided into a substrate and a catalytic domain. Here we present the solution structure of the substrate RNA that is cleaved in a trans reaction by the catalytic domain in the presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix capped by a flexible loop. The cleavage site bulge contains three non-canonical base-pairs, including an A+.C pair with a protonated adenine. This adenine (A622) is a pH controlled conformational switch that opens up the internal loop at higher pH. The possible significance of this switch for substrate recognition and cleavage is discussed.
 ==About this Structure==
-HWQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA].
+HWQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWQ OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Dieckmann, T.]]
-[[Category: Flinders, J.C.]]
+[[Category: Flinders, J C.]]
 [[Category: a+c base pair]]
 [[Category: protonated adenine]]
@@ Line 20: / Line 20: @@
 [[Category: vs ribozyme]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:11:34 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:05:34 2008''