Chloride Intracellular Channel Protein 2: Difference between revisions

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== Structure ==
== Structure ==
[[Image:Nfig001.jpg]]
[[Image:Nfig001.jpg]]
<applet load='2r5g' size='500' frame='true' align='right' caption='Insert caption here' />
<applet load='2r5g' size='500' frame='true' align='right' caption='CLIC2 (2R5G) resolution 1.86 Å' />
Contrary to each members of the CLICs family, CLIC 2 is a monomer, no matter if it is oxydated or reduiced. It is composed of 247 amino acids, has a weight of 28.4kDa and an isoelectric point at 5.44(crystal structure). The CLIC2 molecule is box shaped (60×60×35 Å) and consists of a four strand core and two helices on one side. Comparing sequence similarities, the core is supposed to adopt the canonical fold of the glutathione S-transferase (GST) superfamily. This has been confirmed by the crystal structure determination of human CLIC1 at 1.4 Å resolution. Then, by analyzing CLIC genes sequences, this protein appears to have two potential transmembrane domains that would correspond to helices α1 and α6 in the GST-like structure of the soluble form. Thanks to immunological, electrophysical and proteolysis studies, we can say that membrane form of CLIC proteins cross the lipid bilayer an odd number of times.  
Contrary to each members of the CLICs family, CLIC 2 is a monomer, no matter if it is oxydated or reduiced. It is composed of 247 amino acids, has a weight of 28.4kDa and an isoelectric point at 5.44(crystal structure). The CLIC2 molecule is box shaped (60×60×35 Å) and consists of a four strand core and two helices on one side. Comparing sequence similarities, the core is supposed to adopt the canonical fold of the glutathione S-transferase (GST) superfamily. This has been confirmed by the crystal structure determination of human CLIC1 at 1.4 Å resolution. Then, by analyzing CLIC genes sequences, this protein appears to have two potential transmembrane domains that would correspond to helices α1 and α6 in the GST-like structure of the soluble form. Thanks to immunological, electrophysical and proteolysis studies, we can say that membrane form of CLIC proteins cross the lipid bilayer an odd number of times.  


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== References ==
== References ==
'''CLIC2-RyR1 interaction and structural characterization by cryo-electron microscopy.'''
'''CLIC2-RyR1 interaction and structural characterization by cryo-electron microscopy.'''
Meng X, Wang G, Viero C, Wang Q, Mi W, Su XD, Wagenknecht T, Williams AJ, Liu Z, Yin CC.
Meng X, Wang G, Viero C, Wang Q, Mi W, Su XD, Wagenknecht T, Williams AJ, Liu Z, Yin CC.
J Mol Biol. 2009 Mar.PMID: 19356589  
 
J Mol Biol. 2009 Mar.PMID: 19356589
'''5,5'-Dithio-bis(2-nitrobenzoic acid) modification of cysteine improves the crystal quality of human chloride intracellular channel protein 2.'''
'''5,5'-Dithio-bis(2-nitrobenzoic acid) modification of cysteine improves the crystal quality of human chloride intracellular channel protein 2.'''
Mi W, Li L, Su XD.
Mi W, Li L, Su XD.
Biochem Biophys Res Commun. 2008 Apr 18. PMID: 18280248
Biochem Biophys Res Commun. 2008 Apr 18. PMID: 18280248
'''The crystal structure of human chloride intracellular channel protein 2: a disulfide bond with functional implications.'''
'''The crystal structure of human chloride intracellular channel protein 2: a disulfide bond with functional implications.'''
Mi W, Liang YH, Li L, Su XD.
Mi W, Liang YH, Li L, Su XD.
Proteins. 2008 Apr. PMID: 18186468  
Proteins. 2008 Apr. PMID: 18186468  
'''Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2).'''
'''Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2).'''
Cromer BA, Gorman MA, Hansen G, Adams JJ, Coggan M, Board PG, Parker MW.
Cromer BA, Gorman MA, Hansen G, Adams JJ, Coggan M, Board PG, Parker MW.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Nov 1.PMID: 18007051  
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Nov 1.PMID: 18007051  
'''Structure of the Janus protein human CLIC2'''
Cromer BA, Gorman MA, Hansen G, Adams JJ, Coggan M, Littler DR, Brown LJ, Mazzanti M, Breit SN, Curmi PM, Dulhunty AF, Board PG, Parker MW.
Cromer BA, Gorman MA, Hansen G, Adams JJ, Coggan M, Littler DR, Brown LJ, Mazzanti M, Breit SN, Curmi PM, Dulhunty AF, Board PG, Parker MW.
J Mol Biol. 2007 Nov 30.PMID: 17945253  
J Mol Biol. 2007 Nov 30.PMID: 17945253  
Blood leukocyte microarrays to diagnose systemic onset juvenile idiopathic arthritis and follow the response to IL-1 blockade.
 
'''Blood leukocyte microarrays to diagnose systemic onset juvenile idiopathic arthritis and follow the response to IL-1 blockade'''
 
Allantaz F, Chaussabel D, Stichweh D, Bennett L, Allman W, Mejias A, Ardura M, Chung W, Smith E, Wise C, Palucka K, Ramilo O, Punaro M, Banchereau J, Pascual V.
Allantaz F, Chaussabel D, Stichweh D, Bennett L, Allman W, Mejias A, Ardura M, Chung W, Smith E, Wise C, Palucka K, Ramilo O, Punaro M, Banchereau J, Pascual V.
J Exp Med. 2007 Sep 3. PMID: 17724127  
J Exp Med. 2007 Sep 3. PMID: 17724127  


A recently identified member of the glutathione transferase structural family modifies cardiac RyR2 substate activity, coupled gating and activation by Ca2+ and ATP.
'''A recently identified member of the glutathione transferase structural family modifies cardiac RyR2 substate activity, coupled gating and activation by Ca2+ and ATP.
'''
Dulhunty AF, Pouliquin P, Coggan M, Gage PW, Board PG.
Dulhunty AF, Pouliquin P, Coggan M, Gage PW, Board PG.
Biochem J. 2005 Aug 15.PMID: 15916532  
Biochem J. 2005 Aug 15.PMID: 15916532  
CLIC-2 modulates cardiac ryanodine receptor Ca2+ release channels.
 
'''CLIC-2 modulates cardiac ryanodine receptor Ca2+ release channels.
'''
Board PG, Coggan M, Watson S, Gage PW, Dulhunty AF.
Board PG, Coggan M, Watson S, Gage PW, Dulhunty AF.
Int J Biochem Cell Biol. 2004 Aug .PMID: 15147738  
Int J Biochem Cell Biol. 2004 Aug .PMID: 15147738  
Interaction of Sedlin with chloride intracellular channel proteins.
 
'''Interaction of Sedlin with chloride intracellular channel proteins.
'''
Fan L, Yu W, Zhu X.
Fan L, Yu W, Zhu X.
FEBS Lett. 2003 Apr 10.PMID: 12681486   
FEBS Lett. 2003 Apr 10.PMID: 12681486   
Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts.
 
'''Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts.
'''
Rønnov-Jessen L, Villadsen R, Edwards JC, Petersen OW.
Rønnov-Jessen L, Villadsen R, Edwards JC, Petersen OW.
Am J Pathol. 2002 Aug .PMID: 12163372  
Am J Pathol. 2002 Aug .PMID: 12163372  
Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli.
 
I'''dentification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli.
'''
Berryman M, Bretscher A.
Berryman M, Bretscher A.
Mol Biol Cell. 2000 May.PMID: 10793131  
Mol Biol Cell. 2000 May.PMID: 10793131  


Genomic structure of a novel chloride channel gene, CLIC2, in Xq28.
'''Genomic structure of a novel chloride channel gene, CLIC2, in Xq28.
'''
Heiss NS, Poustka A.
Heiss NS, Poustka A.
Genomics. 1997 Oct 1.PMID: 9339381
Genomics. 1997 Oct 1.PMID: 9339381
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