1dlp: Difference between revisions
New page: left|200px<br /><applet load="1dlp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dlp, resolution 3.3Å" /> '''STRUCTURAL CHARACTERI... |
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[[Image:1dlp.gif|left|200px]]<br /><applet load="1dlp" size=" | [[Image:1dlp.gif|left|200px]]<br /><applet load="1dlp" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1dlp, resolution 3.3Å" /> | caption="1dlp, resolution 3.3Å" /> | ||
'''STRUCTURAL CHARACTERIZATION OF THE NATIVE FETUIN-BINDING PROTEIN SCILLA CAMPANULATA AGGLUTININ (SCAFET): A NOVEL TWO-DOMAIN LECTIN'''<br /> | '''STRUCTURAL CHARACTERIZATION OF THE NATIVE FETUIN-BINDING PROTEIN SCILLA CAMPANULATA AGGLUTININ (SCAFET): A NOVEL TWO-DOMAIN LECTIN'''<br /> | ||
==Overview== | ==Overview== | ||
The three-dimensional structure of a 244-residue, multivalent, fetuin-binding lectin, SCAfet, isolated from bluebell (Scilla campanulata) | The three-dimensional structure of a 244-residue, multivalent, fetuin-binding lectin, SCAfet, isolated from bluebell (Scilla campanulata) bulbs, has been solved at 3.3 A resolution by molecular replacement using the coordinates of the 119-residue, mannose-binding lectin, SCAman, also from bluebell bulbs. Unlike most monocot mannose-binding lectins, such as Galanthus nivalis agglutinin from snowdrop bulbs, which fold into a single domain, SCAfet contains two domains with approximately 55% sequence identity, joined by a linker peptide. Both domains are made up of a 12-stranded beta-prism II fold, with three putative carbohydrate-binding sites, one on each subdomain. SCAfet binds to the complex saccharides of various animal glycoproteins but not to simple sugars. | ||
==About this Structure== | ==About this Structure== | ||
1DLP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hyacinthoides_hispanica Hyacinthoides hispanica]. Full crystallographic information is available from [http:// | 1DLP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hyacinthoides_hispanica Hyacinthoides hispanica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DLP OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Hyacinthoides hispanica]] | [[Category: Hyacinthoides hispanica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Allen, A | [[Category: Allen, A K.]] | ||
[[Category: Donovan, M | [[Category: Donovan, M J.]] | ||
[[Category: Peumans, W | [[Category: Peumans, W J.]] | ||
[[Category: Reynolds, C | [[Category: Reynolds, C D.]] | ||
[[Category: Rizkallah, P | [[Category: Rizkallah, P J.]] | ||
[[Category: VanDamme, E | [[Category: VanDamme, E J.M.]] | ||
[[Category: Wright, L | [[Category: Wright, L M.]] | ||
[[Category: beta prism ii fold]] | [[Category: beta prism ii fold]] | ||
[[Category: native]] | [[Category: native]] | ||
[[Category: two-domain lectin]] | [[Category: two-domain lectin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:17:52 2008'' | ||
Revision as of 10:17, 21 February 2008
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STRUCTURAL CHARACTERIZATION OF THE NATIVE FETUIN-BINDING PROTEIN SCILLA CAMPANULATA AGGLUTININ (SCAFET): A NOVEL TWO-DOMAIN LECTIN
Overview
The three-dimensional structure of a 244-residue, multivalent, fetuin-binding lectin, SCAfet, isolated from bluebell (Scilla campanulata) bulbs, has been solved at 3.3 A resolution by molecular replacement using the coordinates of the 119-residue, mannose-binding lectin, SCAman, also from bluebell bulbs. Unlike most monocot mannose-binding lectins, such as Galanthus nivalis agglutinin from snowdrop bulbs, which fold into a single domain, SCAfet contains two domains with approximately 55% sequence identity, joined by a linker peptide. Both domains are made up of a 12-stranded beta-prism II fold, with three putative carbohydrate-binding sites, one on each subdomain. SCAfet binds to the complex saccharides of various animal glycoproteins but not to simple sugars.
About this Structure
1DLP is a Single protein structure of sequence from Hyacinthoides hispanica. Full crystallographic information is available from OCA.
Reference
Structural characterisation of the native fetuin-binding protein Scilla campanulata agglutinin: a novel two-domain lectin., Wright LM, Reynolds CD, Rizkallah PJ, Allen AK, Van Damme EJ, Donovan MJ, Peumans WJ, FEBS Lett. 2000 Feb 18;468(1):19-22. PMID:10683433
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