1dn4: Difference between revisions

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[[Image:1dn4.gif|left|200px]]<br /><applet load="1dn4" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1dn4.gif|left|200px]]<br /><applet load="1dn4" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1dn4, resolution 1.600Å" />

'''SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES'''<br />

==Overview==

Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at, different temperatures (5 degrees C, 18 degrees C and 37 degrees C) in the, absence of divalent cations. The crystals grown at 5 degrees C did not, diffract X-rays, while those grown at 18 degrees C and 37 degrees C did., The oligomer adopts the left-handed ZI conformation in both crystals. The, main difference resides in a more extensive hydration shell in the crystal, grown at high temperature than in the crystal grown at low temperature., The high-temperature crystal displays a spine of hydration running deep in, the minor groove and linking exocyclic O-2 atoms of the pyrimidine rings., In both crystalline forms, a hydrated sodium ion bound to the N-7 of a, guanine ring was found. Strings of water molecules bridging phosphate, anionic oxygen atoms are found along the backbone. The absolute values of, the propeller-twist are also different in both structures although the, values of the twist are very similar. The results point to the importance, of the crystallization conditions when analysing fine structural details, like solvation properties of oligomers.

Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at different temperatures (5 degrees C, 18 degrees C and 37 degrees C) in the absence of divalent cations. The crystals grown at 5 degrees C did not diffract X-rays, while those grown at 18 degrees C and 37 degrees C did. The oligomer adopts the left-handed ZI conformation in both crystals. The main difference resides in a more extensive hydration shell in the crystal grown at high temperature than in the crystal grown at low temperature. The high-temperature crystal displays a spine of hydration running deep in the minor groove and linking exocyclic O-2 atoms of the pyrimidine rings. In both crystalline forms, a hydrated sodium ion bound to the N-7 of a guanine ring was found. Strings of water molecules bridging phosphate anionic oxygen atoms are found along the backbone. The absolute values of the propeller-twist are also different in both structures although the values of the twist are very similar. The results point to the importance of the crystallization conditions when analysing fine structural details like solvation properties of oligomers.

==About this Structure==

1DN4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1DN4 OCA].

1DN4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DN4 OCA].

==Reference==

Line 13:

[[Category: Protein complex]]

[[Category: Chevrier, B.]]

[[Category: Dock, A.C.]]

[[Category: Dock, A C.]]

[[Category: Hartmann, B.]]

[[Category: Leng, M.]]

[[Category: Moras, D.]]

[[Category: Thuong, M.T.]]

[[Category: Thuong, M T.]]

[[Category: Westhof, E.]]

[[Category: double helix]]

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[[Category: z-dna]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 03~~:20:~~58 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:18:21 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1dn4.gif|left|200px]]<br /><applet load="1dn4" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1dn4.gif|left|200px]]<br /><applet load="1dn4" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1dn4, resolution 1.600&Aring;" />
 '''SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES'''<br />
 ==Overview==
-Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at, different temperatures (5 degrees C, 18 degrees C and 37 degrees C) in the, absence of divalent cations. The crystals grown at 5 degrees C did not, diffract X-rays, while those grown at 18 degrees C and 37 degrees C did., The oligomer adopts the left-handed ZI conformation in both crystals. The, main difference resides in a more extensive hydration shell in the crystal, grown at high temperature than in the crystal grown at low temperature., The high-temperature crystal displays a spine of hydration running deep in, the minor groove and linking exocyclic O-2 atoms of the pyrimidine rings., In both crystalline forms, a hydrated sodium ion bound to the N-7 of a, guanine ring was found. Strings of water molecules bridging phosphate, anionic oxygen atoms are found along the backbone. The absolute values of, the propeller-twist are also different in both structures although the, values of the twist are very similar. The results point to the importance, of the crystallization conditions when analysing fine structural details, like solvation properties of oligomers.
+Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at different temperatures (5 degrees C, 18 degrees C and 37 degrees C) in the absence of divalent cations. The crystals grown at 5 degrees C did not diffract X-rays, while those grown at 18 degrees C and 37 degrees C did. The oligomer adopts the left-handed ZI conformation in both crystals. The main difference resides in a more extensive hydration shell in the crystal grown at high temperature than in the crystal grown at low temperature. The high-temperature crystal displays a spine of hydration running deep in the minor groove and linking exocyclic O-2 atoms of the pyrimidine rings. In both crystalline forms, a hydrated sodium ion bound to the N-7 of a guanine ring was found. Strings of water molecules bridging phosphate anionic oxygen atoms are found along the backbone. The absolute values of the propeller-twist are also different in both structures although the values of the twist are very similar. The results point to the importance of the crystallization conditions when analysing fine structural details like solvation properties of oligomers.
 ==About this Structure==
-DN4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DN4 OCA].
+DN4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DN4 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Protein complex]]
 [[Category: Chevrier, B.]]
-[[Category: Dock, A.C.]]
+[[Category: Dock, A C.]]
 [[Category: Hartmann, B.]]
 [[Category: Leng, M.]]
 [[Category: Moras, D.]]
-[[Category: Thuong, M.T.]]
+[[Category: Thuong, M T.]]
 [[Category: Westhof, E.]]
 [[Category: double helix]]
@@ Line 23: / Line 23: @@
 [[Category: z-dna]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:20:58 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:18:21 2008''