1q3i: Difference between revisions
New page: left|200px<br /><applet load="1q3i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q3i, resolution 2.6Å" /> '''Crystal Structure of ... |
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[[Image:1q3i.jpg|left|200px]]<br /><applet load="1q3i" size=" | [[Image:1q3i.jpg|left|200px]]<br /><applet load="1q3i" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1q3i, resolution 2.6Å" /> | caption="1q3i, resolution 2.6Å" /> | ||
'''Crystal Structure of Na,K-ATPase N-domain'''<br /> | '''Crystal Structure of Na,K-ATPase N-domain'''<br /> | ||
==Overview== | ==Overview== | ||
The structure of the N-domain of porcine alpha(2) Na,K-ATPase was | The structure of the N-domain of porcine alpha(2) Na,K-ATPase was determined crystallographically to 3.2A resolution by isomorphous heavy-atom replacement using a single mercury derivative. The structure was finally refined against 2.6A resolution synchrotron data. The domain forms a seven-stranded antiparallel beta-sheet with two additional beta-strands forming a hairpin and five alpha-helices. Approximately 75% of the residues were superimposable with residues from the structure of Ca-ATPase N-domain, and a structure-based sequence alignment is presented. The positions of key residues are discussed in relation to the pattern of hydrophobicity, charge and sequence conservation of the molecular surface. The structure of a hexahistidine tag binding to nickel ions is presented. | ||
==About this Structure== | ==About this Structure== | ||
1Q3I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Sodium/potassium-exchanging_ATPase Sodium/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.9 3.6.3.9] Full crystallographic information is available from [http:// | 1Q3I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Sodium/potassium-exchanging_ATPase Sodium/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.9 3.6.3.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q3I OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Sodium/potassium-exchanging ATPase]] | [[Category: Sodium/potassium-exchanging ATPase]] | ||
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
[[Category: Hakansson, K | [[Category: Hakansson, K O.]] | ||
[[Category: NI]] | [[Category: NI]] | ||
[[Category: anti-parallel beta sheet]] | [[Category: anti-parallel beta sheet]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:35:33 2008'' | ||
Revision as of 12:35, 21 February 2008
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Crystal Structure of Na,K-ATPase N-domain
Overview
The structure of the N-domain of porcine alpha(2) Na,K-ATPase was determined crystallographically to 3.2A resolution by isomorphous heavy-atom replacement using a single mercury derivative. The structure was finally refined against 2.6A resolution synchrotron data. The domain forms a seven-stranded antiparallel beta-sheet with two additional beta-strands forming a hairpin and five alpha-helices. Approximately 75% of the residues were superimposable with residues from the structure of Ca-ATPase N-domain, and a structure-based sequence alignment is presented. The positions of key residues are discussed in relation to the pattern of hydrophobicity, charge and sequence conservation of the molecular surface. The structure of a hexahistidine tag binding to nickel ions is presented.
About this Structure
1Q3I is a Protein complex structure of sequences from Sus scrofa with NI as ligand. Active as Sodium/potassium-exchanging ATPase, with EC number 3.6.3.9 Full crystallographic information is available from OCA.
Reference
The crystallographic structure of Na,K-ATPase N-domain at 2.6A resolution., Hakansson KO, J Mol Biol. 2003 Oct 3;332(5):1175-82. PMID:14499619
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