1m24: Difference between revisions
New page: left|200px<br /><applet load="1m24" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m24, resolution 0.90Å" /> '''Trichotoxin_A50E, An... |
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[[Image:1m24.jpg|left|200px]]<br /><applet load="1m24" size=" | [[Image:1m24.jpg|left|200px]]<br /><applet load="1m24" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1m24, resolution 0.90Å" /> | caption="1m24, resolution 0.90Å" /> | ||
'''Trichotoxin_A50E, An Ion Channel-Forming Polypeptide'''<br /> | '''Trichotoxin_A50E, An Ion Channel-Forming Polypeptide'''<br /> | ||
==Overview== | ==Overview== | ||
Trichotoxin_A50E is an 18-residue peptaibol antibiotic which forms | Trichotoxin_A50E is an 18-residue peptaibol antibiotic which forms multimeric transmembrane channels through self-association. The crystal structure of trichotoxin has been determined at a resolution of 0.9 A. The trichotoxin sequence contains nine helix-promoting Aib residues, which contribute to the formation of an entirely helical structure that has a central bend of 8-10 degrees located between residues 10-13. Trichotoxin is the first solved structure of the peptaibol family that is all alpha-helix as opposed to containing part or all 3(10)-helix. Gln residues in positions 6 and 17 produce a polar face, and are proposed to form the channel lumen. An octameric model channel has been constructed from the crystal structure. It has a central pore of approximately 4-5 A radius, a size sufficient to enable transport of ions, with a constricted region at one end, formed by a ring of Gln6 residues. Electrostatic calculations are consistent with it being a cationic channel. | ||
==About this Structure== | ==About this Structure== | ||
1M24 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Trichoderma_viride Trichoderma viride] with ACE and CCN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1M24 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Trichoderma_viride Trichoderma viride] with <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=CCN:'>CCN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M24 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Trichoderma viride]] | [[Category: Trichoderma viride]] | ||
[[Category: Brueckner, H.]] | [[Category: Brueckner, H.]] | ||
[[Category: Chugh, J | [[Category: Chugh, J K.]] | ||
[[Category: Wallace, B | [[Category: Wallace, B A.]] | ||
[[Category: ACE]] | [[Category: ACE]] | ||
[[Category: CCN]] | [[Category: CCN]] | ||
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[[Category: peptaibol]] | [[Category: peptaibol]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:50:41 2008'' | ||
Revision as of 11:50, 21 February 2008
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Trichotoxin_A50E, An Ion Channel-Forming Polypeptide
Overview
Trichotoxin_A50E is an 18-residue peptaibol antibiotic which forms multimeric transmembrane channels through self-association. The crystal structure of trichotoxin has been determined at a resolution of 0.9 A. The trichotoxin sequence contains nine helix-promoting Aib residues, which contribute to the formation of an entirely helical structure that has a central bend of 8-10 degrees located between residues 10-13. Trichotoxin is the first solved structure of the peptaibol family that is all alpha-helix as opposed to containing part or all 3(10)-helix. Gln residues in positions 6 and 17 produce a polar face, and are proposed to form the channel lumen. An octameric model channel has been constructed from the crystal structure. It has a central pore of approximately 4-5 A radius, a size sufficient to enable transport of ions, with a constricted region at one end, formed by a ring of Gln6 residues. Electrostatic calculations are consistent with it being a cationic channel.
About this Structure
1M24 is a Protein complex structure of sequences from Trichoderma viride with ACE and CCN as ligands. Full crystallographic information is available from OCA.
Reference
Model for a helical bundle channel based on the high-resolution crystal structure of trichotoxin_A50E., Chugh JK, Bruckner H, Wallace BA, Biochemistry. 2002 Oct 29;41(43):12934-41. PMID:12390019
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