Reverse transcriptase: Difference between revisions

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Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, in company of [[Hiv protease|Protease]] and [[Hiv integrase|Integrase]], the most important part of the protein system involved in the process of infection of viruses like HIV [http://rufusrajadurai.wetpaint.com/video/4026874/Hiv+Replication+3d+Medical+Animation], MuLV and AMV, and has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology.

Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme.

Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme and important tool for genetic engineering applications like RT-PCR in the construction of cDNA libraries.

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==Function==

As a RNA-dependent DNA Polymerase, is able to recognize the initial RNA, transcribe it to ssDNA, cleave the remaining RNA and then build up the dsDNA. Chain A has the polymerase active site that consist of two ''~~finger~~-like'' domains, one of them recognizes the initial nucleic acid possibly by h-bonds interactions with phosphate groups of the side chains, then both domains make a conformational change closing the recognition hole to allow the second domain begin the transcription process. As the same rate that this process occur, the other active site

As a RNA-dependent DNA Polymerase, is able to recognize the initial RNA, transcribe it to ssDNA, cleave the remaining RNA and then build up the dsDNA. Chain A has the polymerase active site that consist of two <scene name='Reverse_transcriptase/Fingers/3'>Finger-like</scene> domains, one of them recognizes the initial nucleic acid possibly by h-bonds interactions with phosphate groups of the side chains, then both domains make a conformational change closing the recognition hole to allow the second domain begin the transcription process. As the same rate that this process occur, the other active site known as the

==See Also==

Reverse transcriptase: Difference between revisions

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@@ Line 1: / Line 1: @@
 <applet load='3jyt' size='350' color='white' frame='true' align='right' caption='Reverse Transcriptase' scene='Reverse_transcriptase/Initial/1' />
 Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, in company of [[Hiv protease|Protease]] and [[Hiv integrase|Integrase]], the most important part of the protein system involved in the process of infection of viruses like HIV [http://rufusrajadurai.wetpaint.com/video/4026874/Hiv+Replication+3d+Medical+Animation], MuLV and AMV, and has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology.
-Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme.
+Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme and important tool for genetic engineering applications like RT-PCR in the construction of cDNA libraries.
@@ Line 8: / Line 8: @@
 ==Function==
-As a RNA-dependent DNA Polymerase, is able to recognize the initial RNA, transcribe it to ssDNA, cleave the remaining RNA and then build up the dsDNA. Chain A has the polymerase active site that consist of two ''finger-like'' domains, one of them recognizes the initial nucleic acid possibly by h-bonds interactions with phosphate groups of the side chains, then both domains make a conformational change closing the recognition hole to allow the second domain begin the transcription process. As the same rate that this process occur, the other active site
+As a RNA-dependent DNA Polymerase, is able to recognize the initial RNA, transcribe it to ssDNA, cleave the remaining RNA and then build up the dsDNA. Chain A has the polymerase active site that consist of two <scene name='Reverse_transcriptase/Fingers/3'>Finger-like</scene> domains, one of them recognizes the initial nucleic acid possibly by h-bonds interactions with phosphate groups of the side chains, then both domains make a conformational change closing the recognition hole to allow the second domain begin the transcription process. As the same rate that this process occur, the other active site known as the
 ==See Also==