Reverse transcriptase: Difference between revisions

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==Function==

As a RNA-dependent DNA Polymerase, is able to recognize the initial RNA, transcribe it to ssDNA, cleave the remaining RNA and then build up the dsDNA. Chain A has the ~~polymerase active site that consist of two~~ <scene name='Reverse_transcriptase/Fingers/3'>~~Finger-like~~</scene> domains, one of them recognizes the initial nucleic acid possibly by h-bonds interactions with phosphate groups of the side chains, then both domains make a conformational change closing the recognition hole to allow the second domain begin the transcription process. As the same rate that this process occur, the other active site known as the

As a RNA-dependent DNA Polymerase, is able to recognize the initial RNA, transcribe it to ssDNA, cleave the remaining RNA and then build up the dsDNA, to do this the protein has two active catalytic zones. Chain A has the <scene name='Reverse_transcriptase/Fingers/1'>Polymerase active site</scene> that consist of two ''finger-like'' domains, one of them recognizes the initial nucleic acid possibly by h-bonds interactions with phosphate groups of the side chains, then both domains make a conformational change closing the recognition hole to allow the second domain begin the transcription process. As the same rate that this process occur, the other active site known as the Ribonuclease H domain, cleaves the RNA releasing the ssDNA that comes again trough the Polymerase active site to become dsDNA.

==See Also==

Reverse transcriptase: Difference between revisions

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 ==Function==
-As a RNA-dependent DNA Polymerase, is able to recognize the initial RNA, transcribe it to ssDNA, cleave the remaining RNA and then build up the dsDNA. Chain A has the polymerase active site that consist of two <scene name='Reverse_transcriptase/Fingers/3'>Finger-like</scene> domains, one of them recognizes the initial nucleic acid possibly by h-bonds interactions with phosphate groups of the side chains, then both domains make a conformational change closing the recognition hole to allow the second domain begin the transcription process. As the same rate that this process occur, the other active site known as the
+As a RNA-dependent DNA Polymerase, is able to recognize the initial RNA, transcribe it to ssDNA, cleave the remaining RNA and then build up the dsDNA, to do this the protein has two active catalytic zones. Chain A has the <scene name='Reverse_transcriptase/Fingers/1'>Polymerase active site</scene> that consist of two ''finger-like'' domains, one of them recognizes the initial nucleic acid possibly by h-bonds interactions with phosphate groups of the side chains, then both domains make a conformational change closing the recognition hole to allow the second domain begin the transcription process. As the same rate that this process occur, the other active site known as the Ribonuclease H domain, cleaves the RNA releasing the ssDNA that comes again trough the Polymerase active site to become dsDNA.
 ==See Also==