1m55: Difference between revisions
New page: left|200px<br /><applet load="1m55" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m55, resolution 1.40Å" /> '''Catalytic domain of ... |
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[[Image:1m55.gif|left|200px]]<br /><applet load="1m55" size=" | [[Image:1m55.gif|left|200px]]<br /><applet load="1m55" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1m55, resolution 1.40Å" /> | caption="1m55, resolution 1.40Å" /> | ||
'''Catalytic domain of the Adeno Associated Virus type 5 Rep protein'''<br /> | '''Catalytic domain of the Adeno Associated Virus type 5 Rep protein'''<br /> | ||
==Overview== | ==Overview== | ||
Adeno-associated virus (AAV), unique among animal viruses in its ability | Adeno-associated virus (AAV), unique among animal viruses in its ability to integrate into a specific chromosomal location, is a promising vector for human gene therapy. AAV Replication (Rep) protein is essential for viral replication and integration, and its amino terminal domain possesses site-specific DNA binding and endonuclease activities required for replication initiation and integration. This domain displays a novel endonuclease fold and demonstrates an unexpected structural relationship to other viral origin binding proteins such as the papillomavirus E1 protein and the SV40 T antigen. The active site, located at the bottom of a positively charged cleft, is formed by the spatial convergence of a divalent metal ion and two conserved sequence motifs that define the rolling circle replication superfamily. | ||
==About this Structure== | ==About this Structure== | ||
1M55 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5] with ZN and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1M55 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus_-_5 Adeno-associated virus - 5] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M55 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dyda, F.]] | [[Category: Dyda, F.]] | ||
[[Category: Hickman, A | [[Category: Hickman, A B.]] | ||
[[Category: Kotin, R | [[Category: Kotin, R M.]] | ||
[[Category: Ronning, D | [[Category: Ronning, D R.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: rolling circle replication]] | [[Category: rolling circle replication]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:51:38 2008'' | ||
Revision as of 11:51, 21 February 2008
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Catalytic domain of the Adeno Associated Virus type 5 Rep protein
Overview
Adeno-associated virus (AAV), unique among animal viruses in its ability to integrate into a specific chromosomal location, is a promising vector for human gene therapy. AAV Replication (Rep) protein is essential for viral replication and integration, and its amino terminal domain possesses site-specific DNA binding and endonuclease activities required for replication initiation and integration. This domain displays a novel endonuclease fold and demonstrates an unexpected structural relationship to other viral origin binding proteins such as the papillomavirus E1 protein and the SV40 T antigen. The active site, located at the bottom of a positively charged cleft, is formed by the spatial convergence of a divalent metal ion and two conserved sequence motifs that define the rolling circle replication superfamily.
About this Structure
1M55 is a Single protein structure of sequence from Adeno-associated virus - 5 with ZN and CL as ligands. Full crystallographic information is available from OCA.
Reference
Structural unity among viral origin binding proteins: crystal structure of the nuclease domain of adeno-associated virus Rep., Hickman AB, Ronning DR, Kotin RM, Dyda F, Mol Cell. 2002 Aug;10(2):327-37. PMID:12191478
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