1qaz: Difference between revisions
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New page: left|200px<br /><applet load="1qaz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qaz, resolution 1.78Å" /> '''CRYSTAL STRUCTURE OF... |
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[[Image:1qaz.gif|left|200px]]<br /><applet load="1qaz" size=" | [[Image:1qaz.gif|left|200px]]<br /><applet load="1qaz" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1qaz, resolution 1.78Å" /> | caption="1qaz, resolution 1.78Å" /> | ||
'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
The three-dimensional structure of alginate lyase A1-III (ALYIII) from a | The three-dimensional structure of alginate lyase A1-III (ALYIII) from a Sphingomonas species A1 was determined by X-ray crystallography. The enzyme was crystallized by the hanging-drop vapour-diffusion method in the presence of 49% ammonium sulfate at 20 degrees C. The crystals are monoclinic and belong to the space group C2 with unit cell dimensions of a=49.18 A, b=93.08 A, c=82.10 A and beta=104.12 degrees. There was one molecule of alginate lyase in the asymmetric unit of the crystal. The diffraction data up to 1. 71 A were collected with Rsymof 5.0%. The crystal structure of ALYIII was solved by the multiple isomorphous replacement method and refined at 1.78 A resolution using X-PLOR with a final R -factor of 18.0% for 10.0 to 1.78 A resolution data. The refined model of ALYIII contained 351 amino acid residues, 299 water molecules and two sulfate ions. The three-dimensional structure of ALYIII was abundant in helices and had a deep tunnel-like cleft in a novel (alpha6/alpha5)-barrel structure, which was similar to the (alpha6/alpha6)-barrel found in glucoamylase and cellulase. This structure presented the possibility that alginate molecules might penetrate into the cleft to interact with the catalytic site of ALYIII. | ||
==About this Structure== | ==About this Structure== | ||
1QAZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp. Sphingomonas sp.] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Urea_carboxylase Urea carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.6 6.3.4.6] Full crystallographic information is available from [http:// | 1QAZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp. Sphingomonas sp.] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Urea_carboxylase Urea carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.6 6.3.4.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QAZ OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Sphingomonas sp.]] | [[Category: Sphingomonas sp.]] | ||
[[Category: Urea carboxylase]] | [[Category: Urea carboxylase]] | ||
[[Category: Yoon, H | [[Category: Yoon, H J.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: alginate lyase]] | [[Category: alginate lyase]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:37:48 2008'' | ||
