1ql5: Difference between revisions
New page: left|200px<br /><applet load="1ql5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ql5" /> '''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADD... |
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[[Image:1ql5.gif|left|200px]]<br /><applet load="1ql5" size=" | [[Image:1ql5.gif|left|200px]]<br /><applet load="1ql5" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT'''<br /> | '''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT'''<br /> | ||
==Overview== | ==Overview== | ||
The cis - syn dimer is the major DNA photoproduct produced by UV | The cis - syn dimer is the major DNA photoproduct produced by UV irradiation. In order to determine the origin of the mutagenic property of the cis - syn dimer, we used NMR restraints and molecular dynamics to determine the solution structure of a DNA decamer duplex containing a wobble pair between the 3'-T of the cis - syn dimer and the opposite T residue (CS/TA duplex). The solution structure of the CS/TA duplex revealed that the 3'-T x T base pair of the cis - syn dimer had base pair geometry that was significantly different from the canonical Watson-Crick base pair and caused destabilization and conformational distortion of its 3'-region. However, a 3'-T x A base pair at the cis - syn dimer within this related DNA decamer maintains the normal Watson-Crick base pair geometry and causes little distortion in the conformation of its 3'-side. Our results show that in spite of its stable hydrogen bonding, the insertion of a T residue opposite the 3'-T of the cis - syn dimer is inhibited by structural distortion caused by the 3'-T x T base pair. This may explain why the frequency of the 3'-T-->A transversion, which is the major mutation produced by the cis - syn dimer, is only 4%. | ||
==About this Structure== | ==About this Structure== | ||
1QL5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1QL5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL5 OCA]. | ||
==Reference== | ==Reference== | ||
Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer., Lee JH, Choi YJ, Choi BS, Nucleic Acids Res. 2000 Apr 15;28(8):1794-801. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10734199 10734199] | Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer., Lee JH, Choi YJ, Choi BS, Nucleic Acids Res. 2000 Apr 15;28(8):1794-801. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10734199 10734199] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Choi, B | [[Category: Choi, B S.]] | ||
[[Category: Hwang, G | [[Category: Hwang, G S.]] | ||
[[Category: Lee, J | [[Category: Lee, J H.]] | ||
[[Category: (6-4) adduct]] | [[Category: (6-4) adduct]] | ||
[[Category: deoxyribonucleic acid]] | [[Category: deoxyribonucleic acid]] | ||
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[[Category: nmr]] | [[Category: nmr]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:40:49 2008'' | ||
Revision as of 12:40, 21 February 2008
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DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT
Overview
The cis - syn dimer is the major DNA photoproduct produced by UV irradiation. In order to determine the origin of the mutagenic property of the cis - syn dimer, we used NMR restraints and molecular dynamics to determine the solution structure of a DNA decamer duplex containing a wobble pair between the 3'-T of the cis - syn dimer and the opposite T residue (CS/TA duplex). The solution structure of the CS/TA duplex revealed that the 3'-T x T base pair of the cis - syn dimer had base pair geometry that was significantly different from the canonical Watson-Crick base pair and caused destabilization and conformational distortion of its 3'-region. However, a 3'-T x A base pair at the cis - syn dimer within this related DNA decamer maintains the normal Watson-Crick base pair geometry and causes little distortion in the conformation of its 3'-side. Our results show that in spite of its stable hydrogen bonding, the insertion of a T residue opposite the 3'-T of the cis - syn dimer is inhibited by structural distortion caused by the 3'-T x T base pair. This may explain why the frequency of the 3'-T-->A transversion, which is the major mutation produced by the cis - syn dimer, is only 4%.
About this Structure
1QL5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer., Lee JH, Choi YJ, Choi BS, Nucleic Acids Res. 2000 Apr 15;28(8):1794-801. PMID:10734199
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