1mis: Difference between revisions
New page: left|200px<br /><applet load="1mis" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mis" /> '''STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*... |
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'''STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | '''STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
The three-dimensional solution structure of the RNA self-complementary | The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities. | ||
==About this Structure== | ==About this Structure== | ||
1MIS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1MIS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIS OCA]. | ||
==Reference== | ==Reference== | ||
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8703939 8703939] | Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8703939 8703939] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Turner, D | [[Category: Turner, D H.]] | ||
[[Category: Wu, M.]] | [[Category: Wu, M.]] | ||
[[Category: ribonucleic acid]] | [[Category: ribonucleic acid]] | ||
| Line 18: | Line 18: | ||
[[Category: tandem g:a mismatch]] | [[Category: tandem g:a mismatch]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:55:32 2008'' | ||
Revision as of 11:55, 21 February 2008
|
STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities.
About this Structure
1MIS is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:8703939
Page seeded by OCA on Thu Feb 21 13:55:32 2008
