1v3n: Difference between revisions

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[[Image:1v3n.gif|left|200px]]<br /><applet load="1v3n" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1v3n.gif|left|200px]]<br /><applet load="1v3n" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1v3n, resolution 1.8Å" />

'''Crystal structure of d(GCGAGAGC): the DNA quadruplex structure split from the octaplex'''<br />

==Overview==

Recent genomic analyses revealed many kinds of tandem repeats of specific, sequences. Some of them are related to genetic diseases, but their, biological functions and structures are still unknown. Two X-ray, structures of a short DNA fragment d(gcGA[G]1Agc) show that four, base-intercalated duplexes are assembled to form an octaplex at a low K+, concentration, in which the eight G5 residues form a stacked double, G-quartet in the central part. At a higher K+ concentration, however, the, octaplex is split into just two halves. These structural features suggest, a folding process of eight tandem repeats of d(ccGA[G]4Agg), according to, a double Greek-key motif. Such a packaging of the repeats could facilitate, slippage of a certain sequence during DNA replication, to induce increase, or decrease of the repeats.

Recent genomic analyses revealed many kinds of tandem repeats of specific sequences. Some of them are related to genetic diseases, but their biological functions and structures are still unknown. Two X-ray structures of a short DNA fragment d(gcGA[G]1Agc) show that four base-intercalated duplexes are assembled to form an octaplex at a low K+ concentration, in which the eight G5 residues form a stacked double G-quartet in the central part. At a higher K+ concentration, however, the octaplex is split into just two halves. These structural features suggest a folding process of eight tandem repeats of d(ccGA[G]4Agg), according to a double Greek-key motif. Such a packaging of the repeats could facilitate slippage of a certain sequence during DNA replication, to induce increase or decrease of the repeats.

==About this Structure==

1V3N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with K as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1V3N OCA].

1V3N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V3N OCA].

==Reference==

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[[Category: x-ray analysis]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 25 04~~:33:~~56 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:31:09 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1v3n.gif|left|200px]]<br /><applet load="1v3n" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1v3n.gif|left|200px]]<br /><applet load="1v3n" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1v3n, resolution 1.8&Aring;" />
 '''Crystal structure of d(GCGAGAGC): the DNA quadruplex structure split from the octaplex'''<br />
 ==Overview==
-Recent genomic analyses revealed many kinds of tandem repeats of specific, sequences. Some of them are related to genetic diseases, but their, biological functions and structures are still unknown. Two X-ray, structures of a short DNA fragment d(gcGA[G]1Agc) show that four, base-intercalated duplexes are assembled to form an octaplex at a low K+, concentration, in which the eight G5 residues form a stacked double, G-quartet in the central part. At a higher K+ concentration, however, the, octaplex is split into just two halves. These structural features suggest, a folding process of eight tandem repeats of d(ccGA[G]4Agg), according to, a double Greek-key motif. Such a packaging of the repeats could facilitate, slippage of a certain sequence during DNA replication, to induce increase, or decrease of the repeats.
+Recent genomic analyses revealed many kinds of tandem repeats of specific sequences. Some of them are related to genetic diseases, but their biological functions and structures are still unknown. Two X-ray structures of a short DNA fragment d(gcGA[G]1Agc) show that four base-intercalated duplexes are assembled to form an octaplex at a low K+ concentration, in which the eight G5 residues form a stacked double G-quartet in the central part. At a higher K+ concentration, however, the octaplex is split into just two halves. These structural features suggest a folding process of eight tandem repeats of d(ccGA[G]4Agg), according to a double Greek-key motif. Such a packaging of the repeats could facilitate slippage of a certain sequence during DNA replication, to induce increase or decrease of the repeats.
 ==About this Structure==
-V3N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with K as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1V3N OCA].
+V3N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V3N OCA].
 ==Reference==
@@ Line 28: / Line 28: @@
 [[Category: x-ray analysis]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:33:56 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:31:09 2008''