Group:SMART:2010 Pingry SMART Team: Difference between revisions
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A key component of Dr. Banta’s work is engineering AdhD to accept a broad range of substrates. This is a crucial component of his work, because this enzyme will be required to act upon a wide range of substrates when it is used within a practical biofuel cell. Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase demonstrates how an enzyme is specific to certain substrates and therefore help show what might be done to broaden the specificity of an enzyme. The function of this enzyme within the rat liver is to regulate/ inactivate steroid hormones. The enzyme does this is by reducing the steroid’s (testosterone) C3 ketone group. The interactions within the active site and testosterone are very specific, because of the structure and positioning of the residues within the cavity. This information is important, because it will help show what might be done to adhd to broaden its substrate specificity. | A key component of Dr. Banta’s work is engineering AdhD to accept a broad range of substrates. This is a crucial component of his work, because this enzyme will be required to act upon a wide range of substrates when it is used within a practical biofuel cell. Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase demonstrates how an enzyme is specific to certain substrates and therefore help show what might be done to broaden the specificity of an enzyme. The function of this enzyme within the rat liver is to regulate/ inactivate steroid hormones. The enzyme does this is by reducing the steroid’s (testosterone) C3 ketone group. The interactions within the active site and testosterone are very specific, because of the structure and positioning of the residues within the cavity. This information is important, because it will help show what might be done to adhd to broaden its substrate specificity. | ||
<applet load='1LWI_chainA.pdb' size='400' frame='true' align='left' caption='1lwi, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with NADP+ cofactor' scene='2010_Pingry_SMART_Team/1lwi_default/ | <applet load='1LWI_chainA.pdb' size='400' frame='true' align='left' caption='1lwi, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with NADP+ cofactor' scene='2010_Pingry_SMART_Team/1lwi_default/6'/> | ||
====PDB ID: 1lwi, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with NADP+ cofactor==== | ====PDB ID: 1lwi, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with NADP+ cofactor==== | ||
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Dark Grey highlights the beta barrel and helix structure. The barrel consists of eight parallel beta strands and eight anti-parallel alpha helices. The bottom is sealed by two antiparallel beta strands (6-10 and 13-18). | Dark Grey highlights the beta barrel and helix structure. The barrel consists of eight parallel beta strands and eight anti-parallel alpha helices. The bottom is sealed by two antiparallel beta strands (6-10 and 13-18). | ||
<scene name='2010_Pingry_SMART_Team/1lwi_betabarrel/ | <scene name='2010_Pingry_SMART_Team/1lwi_betabarrel/4'> | ||
Click Here to view the Beta barrel in blue and Helices in red.</scene> | Click Here to view the Beta barrel in blue and Helices in red.</scene> | ||
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<scene name='2010_Pingry_SMART_Team/1lwi_loops/1'>Purple and Blue highlight the two solvent exposed loops (Purple: Loop A, Blue: Loop B)</scene> | <scene name='2010_Pingry_SMART_Team/1lwi_loops/1'>Purple and Blue highlight the two solvent exposed loops (Purple: Loop A, Blue: Loop B)</scene> | ||
<scene name='2010_Pingry_SMART_Team/1lwi_default/ | <scene name='2010_Pingry_SMART_Team/1lwi_default/6'>Revert to default scene display</scene> | ||
{{Clear}} | {{Clear}} | ||
<applet load='1AFS_chainA.pdb' size='400' frame='true' align='left' caption='1afs, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with cofactor and testosterone' scene='2010_Pingry_SMART_Team/1afs_default/ | <applet load='1AFS_chainA.pdb' size='400' frame='true' align='left' caption='1afs, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with cofactor and testosterone' scene='2010_Pingry_SMART_Team/1afs_default/5'/> | ||
====PDB ID: 1afs, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with cofactor and testosterone==== | ====PDB ID: 1afs, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with cofactor and testosterone==== | ||
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Dark Gray highlights the beta barrel and helix structure. The barrel consists of eight parallel beta strands and eight antiparallel alpha helices. The bottom is sealed by two antiparallel beta strands (6-10 and 13-18). | <scene name='2010_Pingry_SMART_Team/1afs_default/6'>Dark Gray highlights the beta barrel and helix structure.</scene> The barrel consists of eight parallel beta strands and eight antiparallel alpha helices. The bottom is sealed by two antiparallel beta strands (6-10 and 13-18). | ||
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<scene name='2010_Pingry_SMART_Team/1afs_default/ | <scene name='2010_Pingry_SMART_Team/1afs_default/5'>Revert to default scene display</scene> | ||
{{Clear}} | {{Clear}} | ||
