Group:SMART:2010 Pingry SMART Team: Difference between revisions
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Although it can use NADP+ more efficiently, the active site of xylose reductase has evolved to also utilize NAD+. Glu227, Asn276, and Arg280 all interact with both cofactors but in slightly different ways depending on which cofactor is present. The properties of the residues are perfect to interact with multiple key regions on the NAD+ and NADP+ molecules. | Although it can use NADP+ more efficiently, the active site of xylose reductase has evolved to also utilize NAD+. Glu227, Asn276, and Arg280 all interact with both cofactors but in slightly different ways depending on which cofactor is present. The properties of the residues are perfect to interact with multiple key regions on the NAD+ and NADP+ molecules. | ||
<scene name='2010_Pingry_SMART_Team/1k8c_default/4'>Glu227</scene> changes its interactions with the cofactor depending upon if the cofactor is NAD+ or NADP+, it has water-mediated reaction with the 3-prime alcohol group on the ribose. Similarly, <scene> Arg280 </scene> changes position and interacts differently with the two cofactors. <scene name='2010_Pingry_SMART_Team/1k8c_default/ | <scene name='2010_Pingry_SMART_Team/1k8c_default/4'>Glu227</scene> changes its interactions with the cofactor depending upon if the cofactor is NAD+ or NADP+, it has water-mediated reaction with the 3-prime alcohol group on the ribose. Similarly, <scene> Arg280 </scene> changes position and interacts differently with the two cofactors. <scene name='2010_Pingry_SMART_Team/1k8c_default/13'>Asn276</scene> employs hydrogen bonds with the different cofactors. The relative location on the cofacor differs in NAD+ and NADP+. | ||
Lys274 and Ser275 interact only with to the NADP+ cofactor and change positions (usually turn away) when NAD+ is present. | Lys274 and Ser275 interact only with to the NADP+ cofactor and change positions (usually turn away) when NAD+ is present. | ||
<scene name='2010_Pingry_SMART_Team/1k8c_default/5'>Lys274</scene> interacts with the 2-prime alcohol group on the NADP+ ribose but turns way and does not interact when NAD+ is the cofactor. <scene name='2010_Pingry_SMART_Team/1k8c_default/ | <scene name='2010_Pingry_SMART_Team/1k8c_default/5'>Lys274</scene> interacts with the 2-prime alcohol group on the NADP+ ribose but turns way and does not interact when NAD+ is the cofactor. <scene name='2010_Pingry_SMART_Team/1k8c_default/15'>Ser275</scene> | ||
interacts with an oxygen on the phosphate group on the ribose of NADP+ but similarly to the Lys274, does not interact with the NAD+ cofactor. | interacts with an oxygen on the phosphate group on the ribose of NADP+ but similarly to the Lys274, does not interact with the NAD+ cofactor. | ||
