Pyruvate Kinase: Difference between revisions
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m New page: ==Pyruvate Kinase== This is a test Replace the PDB id (use lowercase!) after the STRUCTURE_ and after PDB= to load and display another structure. {{STRUCTURE_2vgb | PDB=2vgb | SCENE=... |
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==Pyruvate Kinase== | ==Pyruvate Kinase== | ||
This is a | Pyruvate kinase is an enzyme that is involved in glycolysis. Pyruvate kinase’s function is to catalyze the last step of glycolysis; thereby, generating the second ATP of glycolysis and pyruvate. It is able to catalyze this step by transferring the phosphate group from phosphoenolpyruvate (PEP) to ADP. | ||
==Mechanism== | |||
Pyruvate kinase catalyzes the final reaction of glycolysis. It couples the free energy of PEP cleavage to the generation of ATP during the synthesis of the final product, pyruvate. This reaction necessitates both K+ and Mg2+ cations, which has two steps. The first step is the nucleophilic attack of the PEP phosphorous atom by β-phosphoryl oxygen of ADP; this step displaces enolpyruvate while forming ATP. In the second step, enolpyruvate tautomerizes to pyruvate. The formation of a high-energy intermediate by enolase in the 9th reaction of glycolysis allows for the synthesis of ATP in this reaction. Though the hydrolysis of 2PG is insufficient in driving the synthesis of ATP, the dehydration of 2PG allows for such a reaction to occur by forming a high-energy intermediate. The high potential of PEP reflects the large release of energy that occurs with the conversion of enolpyruvate to its keto tautomer, pyruvate. | |||
==Structure== | |||
Class: All Beta Proteins | |||
Fold: PK beta-barrel domain-like | |||
Superfamily: PK beta-barrel domain-like | |||
Family: Pyruvate kinase beta-barrel domain | |||
Protein: Pyruvate Kinase (PK) | |||
Species: Human (Homo sapiens) | |||
Replace the PDB id (use lowercase!) after the STRUCTURE_ and after PDB= to load | Replace the PDB id (use lowercase!) after the STRUCTURE_ and after PDB= to load | ||
and display another structure. | and display another structure. | ||
{{ | {{STRUCTURE_1liu | PDB=1liu | SCENE= }} | ||
