User:Laura Carbone/Sandbox 1: Difference between revisions
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<applet load='1ema' size='400' frame='true' align='center' scene='User:Laura_Carbone/Sandbox_1/Chromophore/5' name='5'/> | <applet load='1ema' size='400' frame='true' align='center' scene='User:Laura_Carbone/Sandbox_1/Chromophore/5' name='5'/> | ||
( | (Green links depict the chromophore of each GFP variant. The applet is currently showing the chromophore of wild-type GFP.) | ||
*GFP (eGFP): contains a mutation at Ser<sup>65</sup> in which the residue is most commonly replaced by Thr, Ala, or Gly. Lacks the 395 nm excitation peak, but still emits light at 508 nm like wild-type GFP. | *GFP (eGFP): contains a mutation at Ser<sup>65</sup> in which the residue is most commonly replaced by Thr, Ala, or Gly. Lacks the 395 nm excitation peak, but still emits light at 508 nm like wild-type GFP. | ||
*<scene name='User:Laura_Carbone/Sandbox_1/Bfp_chromophore/1'>BFP</scene> ([[1bfp]]): blue-shifted GFP that has replaced Tyr<sup>66</sup> with His (Y66H mutation). | *<scene name='User:Laura_Carbone/Sandbox_1/Bfp_chromophore/1'>BFP</scene> ([[1bfp]]): blue-shifted GFP that has replaced Tyr<sup>66</sup> with His (Y66H mutation). | ||
*<scene name='User:Laura_Carbone/Sandbox_1/Cfp_chromophore/1'>CFP</scene> ([[1oxd]]): contains Y66H mutation with an emission spectra between BFP and eGFP. | *<scene name='User:Laura_Carbone/Sandbox_1/Cfp_chromophore/1'>CFP</scene> ([[1oxd]]): contains Y66H mutation with an emission spectra between BFP and eGFP. | ||
*Sapphire: excitation spectra at 495 nm has been suppressed while the 395 nm spectra is retained; still emits light at 508 nm. | *Sapphire: excitation spectra at 495 nm has been suppressed while the 395 nm spectra is retained; still emits light at 508 nm. | ||
*YFP ([[1yfp]]): red-shifted GFP that has replaced | *<scene name='User:Laura_Carbone/Sandbox_1/Yfp_chromophore/1'>YFP</scene> ([[1yfp]]): red-shifted GFP that has replaced <scene name='User:Laura_Carbone/Sandbox_1/Yfp_residue_203/1'>residue 203</scene> with an aromatic amino acid. | ||
====Förster Distances (nm) for Energy Transfer==== | ====Förster Distances (nm) for Energy Transfer==== | ||
