Sandbox 156: Difference between revisions

Revision as of 04:22, 21 March 2010 view source Anthony Daniele (talk \| contribs) 114 edits →Reaction of CAT III ← Older edit		Revision as of 04:23, 21 March 2010 view source Anthony Daniele (talk \| contribs) 114 edits →Reaction of CAT III Newer edit →
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	In the first step of the reaction, Histidine-156 abstracts a proton from the 3-hydroxyl of chloramphenicol, ~~causing~~ a nucleophilic attack from the deprotonated oxygen onto the thioester bond of the acetyl-CoA. The 3-acetylchloramphenicol intermediate then rearranges, non-enzymatically, to a 1-acetylchloramphenicol intermediate. CAT III then causes another acetylation of the now regenerated 3-hydroxyl and 1,3-diacetylchloramphenicol is produced.		In the first step of the reaction, Histidine-156 abstracts a proton from the 3-hydroxyl of chloramphenicol, promoting a nucleophilic attack from the deprotonated oxygen onto the thioester bond of the acetyl-CoA. The 3-acetylchloramphenicol intermediate then rearranges, non-enzymatically, to a 1-acetylchloramphenicol intermediate. CAT III then causes another acetylation of the now regenerated 3-hydroxyl and 1,3-diacetylchloramphenicol is produced.

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