Sandbox 156: Difference between revisions

Revision as of 04:23, 21 March 2010 view source Anthony Daniele (talk \| contribs) 114 edits →Reaction of CAT III ← Older edit		Revision as of 04:26, 21 March 2010 view source Anthony Daniele (talk \| contribs) 114 edits →Reaction of CAT III Newer edit →
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	In the first step of the reaction, Histidine-156 abstracts a proton from the 3-hydroxyl of chloramphenicol, promoting a nucleophilic attack from the deprotonated oxygen onto the thioester bond of the acetyl-CoA. The 3-acetylchloramphenicol ~~intermediate~~ then rearranges, non-enzymatically, to a 1-acetylchloramphenicol ~~intermediate~~. ~~CAT III then causes another acetylation~~ of the ~~now regenerated~~ 3-hydroxyl and 1,3-diacetylchloramphenicol is ~~produced~~.		In the first step of the reaction, Histidine-156 abstracts a proton from the 3-hydroxyl of chloramphenicol, promoting a nucleophilic attack from the deprotonated oxygen onto the thioester bond of the acetyl-CoA. The intermediate produced, 3-acetylchloramphenicol, then rearranges non-enzymatically to 1-acetylchloramphenicol. Regeneration of the 3-hydroxyl allows another CAT III catalyzed nucleophilic attack to another acetyl-CoA and a 1,3-diacetylchloramphenicol product is formed.

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