Sandbox 160: Difference between revisions

Line 10:

A simplified illustration of the net reaction is as follows:

D-glyceraldehyde 3-phosphate + phosphate + NAD+ ---------> 3~~-phospho~~-D-~~glyceroyl phosphate~~ + NADH + H+

D-glyceraldehyde 3-phosphate + phosphate + NAD+ ---------> 1,3 biphospho-D-glycerate + NADH + H+

The Pi that is involved in the reaction functions to attack by phosphorolysis the thioester intermediate that is formed by the substrate on the cysteine reside after NAD+ has been reduced. The attack by Pi on the carbonyl carbon of C1 is simultaneously followed by the replacement of bound NADH for NAD+ so another turn of the cycle can now commence. The final product is released as 1,3 bisphosphoglycerate in which the second Pi molecule has been incorporated.

Line 19:

Line 21:

== Active Site in Detail ==

Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1,Arg231NH1 along with N7N and 02'N if the NAD+ moeity help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH.

Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1, Arg231NH1(these two residues are not highlighted in the below illustration) along with N7N and 02'N of the NAD+ moeity (pink) help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH.

Sandbox 160: Difference between revisions

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 10: / Line 10: @@
 A simplified illustration of the net reaction is as follows:
-      D-glyceraldehyde 3-phosphate + phosphate + NAD+ ---------> 3-phospho-D-glyceroyl phosphate + NADH + H+
+      D-glyceraldehyde 3-phosphate + phosphate + NAD+ ---------> 1,3 biphospho-D-glycerate + NADH + H+
+The Pi that is involved in the reaction functions to attack by phosphorolysis the thioester intermediate that is formed by the substrate on the cysteine reside after NAD+ has been reduced. The attack by Pi on the carbonyl carbon of C1 is simultaneously followed by the replacement of bound NADH for NAD+ so another turn of the cycle can now commence. The final product is released as 1,3 bisphosphoglycerate in which the second Pi molecule has been incorporated.
@@ Line 19: / Line 21: @@
 == Active Site in Detail ==
-Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1,Arg231NH1 along with N7N and 02'N if the NAD+ moeity help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH.
+Once Glyceraldehyde 3-phophate comes into contact with the active site it forms a hydrogen bond through its C2 hydroxyl group to Cys149N. The C1 hydroxyl group of the substrate binds to His176NE2. Additional hydrogen bonds to the phosphate group of the substrate from additional residues such as Thr1790G1, Arg231NH1(these two residues are not highlighted in the below illustration) along with N7N and 02'N of the NAD+ moeity (pink) help stabilize the molecule during the course of the reaction in the active site. The nicotinamide ring of the NAD+ ligand is responsible for orienting the hydrogen atom at C1 towards itself which allows for easier transfer in producing in reducing NAD+ to NADH.