Sandbox 156: Difference between revisions

Line 13:

=== Reaction of CAT III ===

------

Both reactions take place in the ~~binding~~ site of ~~chloramphenicol~~, where acetyl-CoA is tunneled through from the opposing side of the trimer<ref>PMID: 3288984</ref>.

Both reactions take place in the active site of CAT III, where acetyl-CoA is tunneled through from the opposing side of the trimer<ref>PMID: 3288984</ref>.

[[Image:Picture2.jpg|500x400 px|center]]

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===The Active Site===

The active site~~, also the chloramphenicol binding site,~~ catalyzes sequential acetylations of the chloramphenicol molecule. This is initiated by the deprotonation of the 3-hydroxyl of chloramphenicol by <scene name='Sandbox_156/Scene_two/1'>His-195</scene><ref>PMID:2015231</ref>. Numerous hydrogen bonds are present and, importantly, van der Waals interactions between the benzene ring of the <scene name='Sandbox_156/Scene_4/1'>Tyr-25</scene> residue and the imidazole ring. This van der Waals contact may help stabilize side-chain orientations, promoting specificity in the reaction. The acetyl group is already properly positioned after tunneling, and reacts readily without any major structural changes<ref>PMID: 3288984</ref>.

The active site catalyzes sequential acetylations of the chloramphenicol molecule. This is initiated by the deprotonation of the 3-hydroxyl of chloramphenicol by <scene name='Sandbox_156/Scene_two/1'>His-195</scene><ref>PMID:2015231</ref>. Numerous hydrogen bonds are present and, importantly, van der Waals interactions between the benzene ring of the <scene name='Sandbox_156/Scene_4/1'>Tyr-25</scene> residue and the imidazole ring. This van der Waals contact may help stabilize side-chain orientations, promoting specificity in the reaction. The acetyl group is already properly positioned after tunneling, and reacts readily without any major structural changes<ref>PMID: 3288984</ref>.

Ace-coa.gif

Sandbox 156: Difference between revisions

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@@ Line 13: / Line 13: @@
 === Reaction of CAT III ===
 ------
-Both reactions take place in the binding site of chloramphenicol, where acetyl-CoA is tunneled through from the opposing side of the trimer<ref>PMID: 3288984</ref>.
+Both reactions take place in the active site of CAT III, where acetyl-CoA is tunneled through from the opposing side of the trimer<ref>PMID: 3288984</ref>.
 [[Image:Picture2.jpg|500x400 px|center]]
@@ Line 34: / Line 34: @@
 ===The Active Site===
-The active site, also the chloramphenicol binding site, catalyzes sequential acetylations of the chloramphenicol molecule. This is initiated by the deprotonation of the 3-hydroxyl of chloramphenicol by <scene name='Sandbox_156/Scene_two/1'>His-195</scene><ref>PMID:2015231</ref>. Numerous hydrogen bonds are present and, importantly, van der Waals interactions between the benzene ring of the <scene name='Sandbox_156/Scene_4/1'>Tyr-25</scene> residue and the imidazole ring. This van der Waals contact may help stabilize side-chain orientations, promoting specificity in the reaction. The acetyl group is already properly positioned after tunneling, and reacts readily without any major structural changes<ref>PMID: 3288984</ref>.
+The active site catalyzes sequential acetylations of the chloramphenicol molecule. This is initiated by the deprotonation of the 3-hydroxyl of chloramphenicol by <scene name='Sandbox_156/Scene_two/1'>His-195</scene><ref>PMID:2015231</ref>. Numerous hydrogen bonds are present and, importantly, van der Waals interactions between the benzene ring of the <scene name='Sandbox_156/Scene_4/1'>Tyr-25</scene> residue and the imidazole ring. This van der Waals contact may help stabilize side-chain orientations, promoting specificity in the reaction. The acetyl group is already properly positioned after tunneling, and reacts readily without any major structural changes<ref>PMID: 3288984</ref>.
 Ace-coa.gif