Sandbox 154: Difference between revisions

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== Structure ==

=== History of the structure ===

The F-actin protein was discovered by Straub in 1942. The structure was speculated based on a low-resolution x-ray crystallograph found in 1990 by Holmes et al. and over this time, despite the importance of F-actin in eukaryotic cells, this speculated structure was accepted. A higher resolution structure was only recently deposited in the PDB databank in Decemeber 2008 by Oda et al. <ref> Oda T, Iwasa M, Aihara T, Maéda Y, and Narita A. 2009. The nature of the globular-to fibrous actin transition. Nature,457(7228):441-445. PMID: [http://www.ncbi.nlm.nih.gov/pubmed/19158791/ 19158791]</ref>.

=== Domains of F-actin Unit ===

Structure of a unit of F-actin with domains from a single polypeptide chain. Note the cleft between the two domains houses the nucleotide phosphate ligand and the Ca2+ metal ion ligand.

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The cleavage of the gamma-phosphoryl from the bound ATP is a result of a conformational change upon binding that moves the Gln137 residue closer to the ATP-Ca2+ ligand. Release of the inorganic phosphate occurs via the conformational change of the flexible "D-loop" into an ordered alpha-helix<ref>graceffa</ref>.

~~=== History of the structure ===~~

The F-actin protein was discovered by Straub in 1942. The structure was speculated based on a low-resolution x-ray crystallograph found in 1990 by Holmes et al. and over this time, despite the importance of F-actin in eukaryotic cells, this speculated structure was accepted. A higher resolution structure was only recently deposited in the PDB databank in Decemeber 2008 by Oda et al. <ref> Oda T, Iwasa M, Aihara T, Maéda Y, and Narita A. 2009. The nature of the globular-to fibrous actin transition. Nature,457(7228):441-445. PMID: [http://www.ncbi.nlm.nih.gov/pubmed/19158791/ 19158791]</ref>.

=== Polymer F-actin ===

Sandbox 154: Difference between revisions

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@@ Line 17: / Line 17: @@
 == Structure ==
 <applet load='2zwh' size='222' color='black' frame='true' align='left' caption='Filamentous Actin (F-actin)' scene='Sandbox_154/2zwh_black_domains/1'/>
+=== History of the structure ===
+The F-actin protein was discovered by Straub in 1942. The structure was speculated based on a low-resolution x-ray crystallograph found in 1990 by Holmes et al. and over this time, despite the importance of F-actin in eukaryotic cells, this speculated structure was accepted. A higher resolution structure was only recently deposited in the PDB databank in Decemeber 2008 by Oda et al. <ref> Oda T, Iwasa M, Aihara T, Maéda Y, and Narita A. 2009. The nature of the globular-to fibrous actin transition. Nature,457(7228):441-445. PMID: [http://www.ncbi.nlm.nih.gov/pubmed/19158791/ 19158791]</ref>.
 === Domains of F-actin Unit ===
 Structure of a unit of F-actin with domains from a single polypeptide chain. Note the cleft between the two domains houses the nucleotide phosphate ligand and the Ca2+ metal ion ligand.
@@ Line 26: / Line 28: @@
 The cleavage of the gamma-phosphoryl from the bound ATP is a result of a conformational change upon binding that moves the Gln137 residue closer to the ATP-Ca2+ ligand. Release of the inorganic phosphate occurs via the conformational change of the flexible "D-loop" into an ordered alpha-helix<ref>graceffa</ref>.
-=== History of the structure ===
-The F-actin protein was discovered by Straub in 1942. The structure was speculated based on a low-resolution x-ray crystallograph found in 1990 by Holmes et al. and over this time, despite the importance of F-actin in eukaryotic cells, this speculated structure was accepted. A higher resolution structure was only recently deposited in the PDB databank in Decemeber 2008 by Oda et al. <ref> Oda T, Iwasa M, Aihara T, Maéda Y, and Narita A. 2009. The nature of the globular-to fibrous actin transition. Nature,457(7228):441-445. PMID: [http://www.ncbi.nlm.nih.gov/pubmed/19158791/ 19158791]</ref>.
 === Polymer F-actin ===