Green Fluorescent Protein: Difference between revisions
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'''CFP-YFP''': This pair has been used more recently as an alternative to the BFP-eGFP pair because CFP is significantly brighter than BFP, allowing for more accurate ratiometric measurement of donor to acceptor emissions. However, filters must be used carefully while using CFP-YFP in FRET because of the bleeding of the CFP spectra into the YFP spectra. Therefore, proper filters must be set in order to distinguish the fluorescence from donor versus acceptor <ref name="Pollock" />. | '''CFP-YFP''': This pair has been used more recently as an alternative to the BFP-eGFP pair because CFP is significantly brighter than BFP, allowing for more accurate ratiometric measurement of donor to acceptor emissions. However, filters must be used carefully while using CFP-YFP in FRET because of the bleeding of the CFP spectra into the YFP spectra. Therefore, proper filters must be set in order to distinguish the fluorescence from donor versus acceptor <ref name="Pollock" />. | ||
==Related Structures | ==Related Structures== | ||
*[[1ema]] ''Aequorea victoria'' Green Fluorescent Protein, monomer | *[[1ema]] ''Aequorea victoria'' Green Fluorescent Protein, monomer | ||
