1qlb: Difference between revisions
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[[Image:1qlb.jpg|left|200px]]<br /><applet load="1qlb" size=" | [[Image:1qlb.jpg|left|200px]]<br /><applet load="1qlb" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1qlb, resolution 2.33Å" /> | caption="1qlb, resolution 2.33Å" /> | ||
'''RESPIRATORY COMPLEX II-LIKE FUMARATE REDUCTASE FROM WOLINELLA SUCCINOGENES'''<br /> | '''RESPIRATORY COMPLEX II-LIKE FUMARATE REDUCTASE FROM WOLINELLA SUCCINOGENES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1QLB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes] with CA, HEM, FES, F3S, SF4, FAD, FMR and LMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Succinate_dehydrogenase Succinate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.1 1.3.99.1] Known structural/functional Sites: <scene name='pdbsite=FA1:Fad Covalent Bond To Protein For Chain A'>FA1</scene>, <scene name='pdbsite=FA2:Fad Covalent Bond To Protein For Chain D'>FA2</scene>, <scene name='pdbsite=FS1:Fe4s4 Fe Sulphur Centre Ligands For Chain B'>FS1</scene>, <scene name='pdbsite=FS2:Fe4s4 Fe Sulphur Centre Ligands For Chain E'>FS2</scene>, <scene name='pdbsite=FS3:Fe2s2 Fe Sulphur Centre Ligands For Chain B'>FS3</scene>, <scene name='pdbsite=FS4:Fe2s2 Fe Sulphur Centre Ligands For Chain E'>FS4</scene>, <scene name='pdbsite=FS5:Fe3s4 Fe Sulphur Centre Ligands For Chain B'>FS5</scene>, <scene name='pdbsite=FS6:Fe3s4 Fe Sulphur Centre Ligands For Chain E'>FS6</scene>, <scene name='pdbsite=HE1:Haem Axial Ligands For Chain C'>HE1</scene> and <scene name='pdbsite=HE2:Haem Axial Ligands For Chain F'>HE2</scene>. Full crystallographic information is available from [http:// | 1QLB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=FES:'>FES</scene>, <scene name='pdbligand=F3S:'>F3S</scene>, <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=FAD:'>FAD</scene>, <scene name='pdbligand=FMR:'>FMR</scene> and <scene name='pdbligand=LMT:'>LMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Succinate_dehydrogenase Succinate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.1 1.3.99.1] Known structural/functional Sites: <scene name='pdbsite=FA1:Fad+Covalent+Bond+To+Protein+For+Chain+A'>FA1</scene>, <scene name='pdbsite=FA2:Fad+Covalent+Bond+To+Protein+For+Chain+D'>FA2</scene>, <scene name='pdbsite=FS1:Fe4s4+Fe+Sulphur+Centre+Ligands+For+Chain+B'>FS1</scene>, <scene name='pdbsite=FS2:Fe4s4+Fe+Sulphur+Centre+Ligands+For+Chain+E'>FS2</scene>, <scene name='pdbsite=FS3:Fe2s2+Fe+Sulphur+Centre+Ligands+For+Chain+B'>FS3</scene>, <scene name='pdbsite=FS4:Fe2s2+Fe+Sulphur+Centre+Ligands+For+Chain+E'>FS4</scene>, <scene name='pdbsite=FS5:Fe3s4+Fe+Sulphur+Centre+Ligands+For+Chain+B'>FS5</scene>, <scene name='pdbsite=FS6:Fe3s4+Fe+Sulphur+Centre+Ligands+For+Chain+E'>FS6</scene>, <scene name='pdbsite=HE1:Haem+Axial+Ligands+For+Chain+C'>HE1</scene> and <scene name='pdbsite=HE2:Haem+Axial+Ligands+For+Chain+F'>HE2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QLB OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: succinate dehydrogenase]] | [[Category: succinate dehydrogenase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:00:54 2008'' | ||
Revision as of 08:00, 3 February 2008
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RESPIRATORY COMPLEX II-LIKE FUMARATE REDUCTASE FROM WOLINELLA SUCCINOGENES
Overview
Fumarate reductase couples the reduction of fumarate to succinate to the, oxidation of quinol to quinone, in a reaction opposite to that catalysed, by the related complex II of the respiratory chain (succinate, dehydrogenase). Here we describe the crystal structure at 2.2 A resolution, of the three protein subunits containing fumarate reductase from the, anaerobic bacterium Wolinella succinogenes. Subunit A contains the site of, fumarate reduction and a covalently bound flavin adenine dinucleotide, prosthetic group. Subunit B contains three iron-sulphur centres. The, menaquinol-oxidizing subunit C consists of five membrane-spanning, primarily helical segments and binds two haem b molecules. On the basis of, the structure, we propose a pathway of electron transfer from the dihaem, cytochrome b to the site of fumarate reduction and a mechanism of fumarate, reduction. The relative orientations of the soluble and membrane-embedded, subunits of succinate:quinone oxidoreductases appear to be unique.
About this Structure
1QLB is a Protein complex structure of sequences from Wolinella succinogenes with CA, HEM, FES, F3S, SF4, FAD, FMR and LMT as ligands. Active as Succinate dehydrogenase, with EC number 1.3.99.1 Known structural/functional Sites: FA1, FA2, FS1, FS2, FS3, FS4, FS5, FS6, HE1 and HE2. Full crystallographic information is available from OCA.
Reference
Structure of fumarate reductase from Wolinella succinogenes at 2.2 A resolution., Lancaster CR, Kroger A, Auer M, Michel H, Nature. 1999 Nov 25;402(6760):377-85. PMID:10586875
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