P19: Difference between revisions
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siRNAs are generally characterized by their short length (21–26 nt), 2 nt, 3′ overhanging ends, and 5′ phosphate groups. The most efficient silencing is obtained with siRNA duplexes composed of 21-nt sense and 21-nt antisense strands, paired in a manner to have a 2-nt 3' overhang (see [http://www.rockefeller.edu/labheads/tuschl/sirna.html the Tuschl lab's guide] for designing siRNAs)<ref>Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate., Elbashir SM, Martinez J, Patkaniowska A, Lendeckel W, Tuschl T, EMBO J. 2001 Dec 3;20(23):6877-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11726523 11726523]</ref>. | siRNAs are generally characterized by their short length (21–26 nt), 2 nt, 3′ overhanging ends, and 5′ phosphate groups. The most efficient silencing is obtained with siRNA duplexes composed of 21-nt sense and 21-nt antisense strands, paired in a manner to have a 2-nt 3' overhang (see [http://www.rockefeller.edu/labheads/tuschl/sirna.html the Tuschl lab's guide] for designing siRNAs)<ref>Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate., Elbashir SM, Martinez J, Patkaniowska A, Lendeckel W, Tuschl T, EMBO J. 2001 Dec 3;20(23):6877-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11726523 11726523]</ref>. | ||
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Structural studies <ref>Size selective recognition of siRNA by an RNA silencing suppressor., Vargason JM, Szittya G, Burgyan J, Tanaka Hall TM, Cell 2003 Dec 26;115(7):799-811. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14697199 14697199]</ref><ref>Recognition of small interfering RNA by a viral suppressor of RNA silencing., Ye K, Malinina L, Patel D J, Nature 2003 426(6968):874-878. Epub 2003 Dec 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14661029 14661029]</ref> have revealed how p19 selectively recognizes the double-stranded siRNA. | Structural studies <ref>Size selective recognition of siRNA by an RNA silencing suppressor., Vargason JM, Szittya G, Burgyan J, Tanaka Hall TM, Cell 2003 Dec 26;115(7):799-811. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14697199 14697199]</ref><ref>Recognition of small interfering RNA by a viral suppressor of RNA silencing., Ye K, Malinina L, Patel D J, Nature 2003 426(6968):874-878. Epub 2003 Dec 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14661029 14661029]</ref> have revealed how p19 from carnation and tomato viruses selectively recognizes the double-stranded siRNA. p19 from the carnation virus is presented on this page; however the results are applicable to both p19s as the solved structures are very similar, consistent with the high percent of identity (89%) for the homologous proteins<ref name='alignment'>[[Alignment of 1rpu vs. 1r9f]].</ref>. | ||
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==Notes and Literature References== | ==Notes and Literature References== | ||
<references/> | <references/> | ||
==Additional Literature and Resources== | ==Additional Literature and Resources== | ||
*[http://www-unix.oit.umass.edu/~wdecatur/p19tutorial/ Tour of p19 bound to an siRNA] by Wayne Decatur, in an exploration-friendly interface that is adapted from Eric Martz's [http://molvis.sdsc.edu/fgij/index.htm FirstGlance in Jmol]<br> | *[http://www-unix.oit.umass.edu/~wdecatur/p19tutorial/ Tour of p19 bound to an siRNA] by Wayne Decatur, in an exploration-friendly interface that is adapted from Eric Martz's [http://molvis.sdsc.edu/fgij/index.htm FirstGlance in Jmol]<br> | ||
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[[Category: Vargason, J M.]] | [[Category: Vargason, J M.]] | ||
[[Category: Protein-rna complex]] | [[Category: Protein-rna complex]] | ||
[[ | [[Category: Protein complex]] | ||
[[Category: Rna double helix]] | [[Category: Rna double helix]] | ||
[[Category: A-form helix]] | [[Category: A-form helix]] | ||
[[Category: Rna length recognition]] | [[Category: Rna length recognition]] | ||
[[Category: Rnai]] | [[Category: Rnai]] | ||
