2ja5: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2ja5.jpg|left|200px]]<br /><applet load="2ja5" size=" | [[Image:2ja5.jpg|left|200px]]<br /><applet load="2ja5" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2ja5, resolution 3.8Å" /> | caption="2ja5, resolution 3.8Å" /> | ||
'''CPD LESION CONTAINING RNA POLYMERASE II ELONGATION COMPLEX A'''<br /> | '''CPD LESION CONTAINING RNA POLYMERASE II ELONGATION COMPLEX A'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
2JA5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Known structural/functional Site: <scene name='pdbsite=AC1:Zn Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | 2JA5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Known structural/functional Site: <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JA5 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 48: | Line 48: | ||
[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:43:21 2008'' | ||
Revision as of 08:43, 3 February 2008
|
CPD LESION CONTAINING RNA POLYMERASE II ELONGATION COMPLEX A
Overview
Cells use transcription-coupled repair (TCR) to efficiently eliminate DNA, lesions such as ultraviolet light-induced cyclobutane pyrimidine dimers, (CPDs). Here we present the structure-based mechanism for the first step, in eukaryotic TCR, CPD-induced stalling of RNA polymerase (Pol) II. A CPD, in the transcribed strand slowly passes a translocation barrier and enters, the polymerase active site. The CPD 5'-thymine then directs uridine, misincorporation into messenger RNA, which blocks translocation., Artificial replacement of the uridine by adenosine enables CPD bypass;, thus, Pol II stalling requires CPD-directed misincorporation. In the, stalled complex, the lesion is inaccessible, and the polymerase, conformation is unchanged. This is consistent with nonallosteric, recruitment of repair factors and excision of a lesion-containing DNA, fragment in the presence of Pol II.
About this Structure
2JA5 is a Protein complex structure of sequences from Saccharomyces cerevisiae with MG and ZN as ligands. Active as DNA-directed RNA polymerase, with EC number 2.7.7.6 Known structural/functional Site: AC1. Full crystallographic information is available from OCA.
Reference
CPD damage recognition by transcribing RNA polymerase II., Brueckner F, Hennecke U, Carell T, Cramer P, Science. 2007 Feb 9;315(5813):859-62. PMID:17290000
Page seeded by OCA on Sun Feb 3 10:43:21 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- DNA-directed RNA polymerase
- Protein complex
- Saccharomyces cerevisiae
- Brueckner, F.
- Carell, T.
- Cramer, P.
- Hennecke, U.
- MG
- ZN
- Arrest
- Cpd
- Cyclobutane pyrimidine dimer
- Damage recognition
- Dna damage
- Dna lesion
- Dna-binding
- Dna-directed rna polymerase
- Elongation complex
- Lesion recognition
- Metal-binding
- Misincorporation
- Nuclear protein
- Nucleotidyltransferase
- Phosphorylation
- Photolesion
- Rna polymerase ii
- Stalling
- Tcr
- Thymine dimer
- Transcription
- Transcription bubble
- Transcription- coupled repair
- Transferase
- Transferase/dna/rna
- Zinc
- Zinc-finger
