3mp2: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
{{STRUCTURE_3mp2| PDB=3mp2 | SCENE= }} | {{STRUCTURE_3mp2| PDB=3mp2 | SCENE= }} | ||
===Crystal structure of transmissible gastroenteritis virus papain-like protease 1=== | ===Crystal structure of transmissible gastroenteritis virus papain-like protease 1=== | ||
{{ABSTRACT_PUBMED_20668092}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/R1A_CVPPU R1A_CVPPU]] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). | |||
- | |||
==About this Structure== | ==About this Structure== | ||
[[3mp2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Transmissible_gastroenteritis_virus Transmissible gastroenteritis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MP2 OCA]. | |||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID: | <ref group="xtra">PMID:020668092</ref><references group="xtra"/><references/> | ||
[[Category: | [[Category: Transmissible gastroenteritis virus]] | ||
[[Category: Manolaridis, I.]] | [[Category: Manolaridis, I.]] | ||
[[Category: Tucker, P A.]] | [[Category: Tucker, P A.]] | ||
| Line 32: | Line 19: | ||
[[Category: Papain-like protease]] | [[Category: Papain-like protease]] | ||
[[Category: Tgev]] | [[Category: Tgev]] | ||
Revision as of 21:01, 17 April 2013
Crystal structure of transmissible gastroenteritis virus papain-like protease 1
Template:ABSTRACT PUBMED 20668092
Function
[R1A_CVPPU] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SAGC]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).
About this Structure
3mp2 is a 1 chain structure with sequence from Transmissible gastroenteritis virus. Full crystallographic information is available from OCA.
Reference
- Wojdyla JA, Manolaridis I, van Kasteren PB, Kikkert M, Snijder EJ, Gorbalenya AE, Tucker PA. Papain-Like Protease 1 from Transmissible Gastroenteritis Virus: Crystal Structure and Enzymatic Activity toward Viral and Cellular Substrates. J Virol. 2010 Oct;84(19):10063-73. Epub 2010 Jul 28. PMID:20668092 doi:10.1128/JVI.00898-10
