2v79: Difference between revisions

The DnaD protein is an essential component of the chromosome-replication, machinery of the Gram-positive bacterium Bacillus subtilis and is part of, the primosomal cascade that ultimately loads the replicative ring helicase, DnaC onto DNA. Moreover, DnaD is a global regulator of DNA architecture, as it forms higher order nucleoprotein structures in order to open, supercoiled DNA. Here, the crystallization and preliminary X-ray, diffraction analysis of the two domains of DnaD from B. subtilis are, reported. Crystals of the N-terminal domain are trigonal, with either, P3(1)21 or P3(2)21 space-group symmetry, and diffracted X-rays to 2.0 A, resolution; crystals of the C-terminal domain are hexagonal, with space, group P6(1) or P6(5), and diffracted X-rays to 2.9 A resolution in-house., Determination of the structure of the DnaD domains will provide insight, into how remodelling of the nucleoid is associated with priming of, replication in the model Gram-positive organism B. subtilis.

2V79 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Na Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Na Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Na Binding Site For Chain B'>AC3</scene>, <scene name='pdbsite=AC4:Cl Binding Site For Chain B'>AC4</scene>, <scene name='pdbsite=AC5:Cl Binding Site For Chain A'>AC5</scene>, <scene name='pdbsite=AC6:Cl Binding Site For Chain B'>AC6</scene>, <scene name='pdbsite=AC7:Cl Binding Site For Chain B'>AC7</scene>, <scene name='pdbsite=AC8:Cl Binding Site For Chain B'>AC8</scene>, <scene name='pdbsite=AC9:Cl Binding Site For Chain B'>AC9</scene>, <scene name='pdbsite=BC1:Cl Binding Site For Chain A'>BC1</scene>, <scene name='pdbsite=BC2:Cl Binding Site For Chain A'>BC2</scene>, <scene name='pdbsite=BC3:Cl Binding Site For Chain A'>BC3</scene>, <scene name='pdbsite=BC4:Cl Binding Site For Chain B'>BC4</scene> and <scene name='pdbsite=BC5:Cl Binding Site For Chain B'>BC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V79 OCA].  

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